• Circadian rhythms control body’s response to intestinal infections

    Updated: 2013-05-31 18:39:28
    ScienceBlog.com Science news straight from the source Navigation About Our Bloggers Subscribe by Email Topics Brain Behavior Earth , Energy Environment Health Medicine Life Non-humans Physics Mathematics Space Technology Circadian rhythms control body’s response to intestinal infections Circadian rhythms can boost the body’s ability to fight intestinal bacterial infections , UC Irvine researchers have . found This suggests that targeted treatments may be particularly effective for pathogens such as salmonella that prompt a strong immune system response governed by circadian genes . It also helps explain why disruptions in the regular day-night pattern as experienced by , say , night-shift workers or frequent fliers may raise susceptibility to infectious . diseases UC Irvine’s Paolo

  • The scoop on bird poop: The evolving diversity of microbial life in bird guts

    Updated: 2013-05-31 16:42:44
    : ScienceBlog.com Science news straight from the source Navigation About Our Bloggers Subscribe by Email Topics Brain Behavior Earth , Energy Environment Health Medicine Life Non-humans Physics Mathematics Space Technology The scoop on bird poop : The evolving diversity of microbial life in bird guts Gut bacteria are known to have a central role both in human and in animal . health Animals acquire different bacteria as they age but how the microbial communities in the bodies of wild animals change over time is not well understood . Wouter van Dongen and colleagues at the Vetmeduni Vienna have examined the gastrointestinal bacteria of chick and adult black-legged kittiwakes . Surprisingly , the microbial assemblages of chicks and adults generally differ greatly , with only a few types of

  • Investigation of the role of tryptophan residues in cationic antimicrobial peptides to determine the mechanism of antimicrobial action

    Updated: 2013-05-31 09:16:33
    ConclusionsThe position of Trp, rather than the number of Trp residues, in these peptides was an important factor in the antimicrobial activity. Trp residues were deeply inserted into negatively charged membranes but were largely exposed in aqueous buffer solution. Significance and Impact of the StudyThese Trp‐containing peptides may represent good candidates for new antibiotic agents and for use in new therapeutic approaches.This article is protected by copyright. All rights reserved. (Source: Journal of Applied Microbiology)

  • How does a Mycobacterium change its spots? Applying molecular tools to track diverse strains of Mycobacterium avium subspecies paratuberculosis

    Updated: 2013-05-31 09:16:11
    This article is protected by copyright. All rights reserved. (Source: Letters in Applied Microbiology)

  • Transcriptional regulation of fatty acid biosynthesis in mycobacteria

    Updated: 2013-05-31 09:15:49
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Molecular Microbiology Molecular Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES 3D Interactive Images Faculty of 1000 Prizes Awarded by Molecular Microbiology Virtual Special Issues

  • Genetic and antigenic analyses of Porphyromonas gingivalis FimA fimbriae

    Updated: 2013-05-31 09:15:04
    This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Rapid detection of haemotropic mycoplasma infection of feline erythrocytes using a novel flow cytometric approach

    Updated: 2013-05-31 04:00:00
    We report a novel flow cytometric system to identify and monitor infection of mammalian erythrocytes by distinct Mycoplasma species. Image: George, the Siamese cat infected with Mycoplasma hemofelis, receiving a blood transfusion. (Source: Parasites and Vectors)

  • Mycobacterium avium genotype is associated with the therapeutic response to lung infection

    Updated: 2013-05-31 04:00:00
    In conclusion, we identified four mycobacterial minisatellite loci that together were associated with the therapeutic response of M. avium lung infections. This article is protected by copyright. All rights reserved. (Source: Clinical Microbiology and Infection)

  • Oral bacterial community dynamics in paediatric patients with malignancies in relation to chemotherapy‐related oral mucositis: a prospective study

    Updated: 2013-05-31 04:00:00
    In conclusion, we find a higher microbial diversity at the time of malignancy diagnosis in patients that later develop oral mucositis and that these patients have a more significant modification of the bacterial community by chemotherapy before mucositis occurrence. These findings possibly will be of clinical importance for better strategies of personalised preventive management. This article is protected by copyright. All rights reserved. (Source: Clinical Microbiology and Infection)

  • Quantitative insights into actin rearrangements and bacterial target site selection from Salmonella Typhimurium infection of micropatterned cells

    Updated: 2013-05-30 04:00:00
    Summary Reorganization of the host cell actin cytoskeleton is crucial during pathogen invasion. We established micropatterned cells as a standardized infection model for cell invasion to quantitatively study actin rearrangements triggered by Salmonella Typhimurium (S. Tm). Micropatterns of extracellular matrix proteins force cells to adopt a reproducible shape avoiding strong cell‐to‐cell variations, a major limitation in classical cell culture conditions. S. Tm induced F‐actin‐rich ruffles and invaded micropatterned cells similar to unconstrained cells. Yet, standardized conditions allowed fast and unbiased comparison of cellular changes triggered by the SipA and SopE bacterial effector proteins. Intensity measurements in defined regions revealed that the content of pre‐exis...

  • Correlation analysis of Shiga toxin–producing Escherichia coli shedding and faecal bacterial composition in beef cattle

    Updated: 2013-05-30 04:00:00
    ConclusionsHigher STEC shedding levels and prevalence were associated with younger cattle age, lower faecal bacterial diversity and lower BPB levels. Significance and Impact of the StudyButyrate‐producing bacteria in GI tract might serve as an option for the future development of STEC shedding control strategy. (Source: Journal of Applied Microbiology)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Genotypic and phenotypic characterization of methicillin‐resistant Staphylococcus aureus (MRSA) isolated from milk of bovine mastitis

    Updated: 2013-05-30 04:00:00
    This study confirms the presence of ST398 MRSA in milk from bovine mastitis in Belgium. Moreover, the isolated MRSA strains were described for genotypic and phenotypic characteristics potentially implicated in virulence. This study highlights that Belgian bovine could be a reservoir of MRSA for human. Significance and Impact of the Study: This study confirms the presence of ST398 MRSA in milk from bovine mastitis in Belgium. Moreover, the isolated MRSA strains were described for genotypic and phenotypic characteristics potentially implicated in virulence. This study highlights that Belgian bovine could be a reservoir of MRSA for human. (Source: Letters in Applied Microbiology)

  • Interleukin-22 protects against post-influenza bacterial superinfection

    Updated: 2013-05-30 04:00:00
    (American Society for Microbiology) Researchers from the Pasteur Institute, Lille, France have shown in a mouse model that interleukin-22 protects against bacterial superinfections that can arise following influenza. Their research is published in the June 2013 issue of the Journal of Virology. (Source: EurekAlert! - Medicine and Health)

  • New agent inhibits HCV replication in mouse models -- No resistance seen

    Updated: 2013-05-30 04:00:00
    (American Society for Microbiology) Treatments against hepatitis C virus have only been partially successful. A major problem is that antivirals generate drug resistance. Now Seong-Wook Lee of Dankook University, Yongin, Republic of Korea and his collaborators have developed agents that bind to the business end of a critical protein, disabling it so successfully that no resistance has arisen. The research is published in the June 2013 issue of the Journal of Virology. (Source: EurekAlert! - Medicine and Health)

  • Replicating DNA by cell factories: roles of central carbon metabolism and transcription in the control of DNA replication in microbes, and implications for understanding this process in human cells

    Updated: 2013-05-29 04:00:00
    : , Log on BioMed Central Journals Gateways 3.55 Search this journal BioMed Central for Go Advanced search Home Articles Authors Reviewers About this journal My Microbial Cell Factories Microbial Cell Factories Volume 12 Viewing options Abstract Provisional PDF 3.9MB Associated material PubMed record About this article Readers' comments Related literature Cited by Google blog search Other articles by authors on Google Scholar Bara ska S Glinkowska M Herman-Antosiewicz A Maci g-Dorszy ska M Nowicki D Szalewska-Pa asz A W grzyn A W grzyn G on PubMed Bara ska S Glinkowska M Herman-Antosiewicz A Maci g-Dorszy ska M Nowicki D Szalewska-Pa asz A W grzyn A W grzyn G Related articles pages on Google on Google Scholar on PubMed Tools Download references Email to a friend Order reprints

  • Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

    Updated: 2013-05-29 04:00:00
    Conclusions: These data bring new insights into P. mosselii virulence, since this bacterium has often been neglected due to its rare occurrence in hospital. (Source: BMC Microbiology - Latest articles)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Elevated CO2 influences microbial carbon and nitrogen cycling

    Updated: 2013-05-29 04:00:00
    Conclusions: These findings suggest that the soil microbial community structure and their ecosystem functioning for C and N cycling were altered dramatically at eCO2. This study provides new insights into our understanding of the feedback response of soil microbial communities to elevated CO2 and global change. (Source: BMC Microbiology - Latest articles)

  • Molecular cloning of a laccase gene from Ganoderma lucidum and heterologous expression in Pichia pastoris

    Updated: 2013-05-29 04:00:00
    A genomic laccase gene and cDNA were cloned from the white‐rot fungi Ganoderma lucidum TR6. The genomic laccase gene contained 2086 bp with nine introns. The laccase cDNA had an open reading frame of 1563 bp. The deduced mature protein consisted of 520 amino acids. Both the genomic laccase gene and cDNA were expressed in the Pichia pastoris GS115. Laccase activities could be detected in transformants with laccase cDNA but not in transformants with genomic laccase gene. The highest activity value reached 685.8 U L−1. The effects of temperature, pH and nitrogen source on laccase expression in P. pastoris were analyzed. The recombinant laccase was purified and the molecular mass was 73.4 KDa, a little bigger than native laccase. The optimal pH and temperature were specific at pH...

  • Chlorobenzene degradation by Bacillus sp. TAS6CB: A potential candidate to remediate chlorinated hydrocarbon contaminated sites

    Updated: 2013-05-29 04:00:00
    . : Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Journal of Basic Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS Submit an Article OnlineOpen Author Guidelines ABOUT THIS JOURNAL Contact Editorial Board Advertise Overview SPECIAL FEATURES Cover Gallery Read Cover Story Wiley Job Network Research Paper Chlorobenzene degradation by Bacillus sp . TAS6CB : A potential

  • Anthranilate degradation by a cold‐adapted Pseudomonas sp. 

    Updated: 2013-05-29 04:00:00
    An alpine soil bacterium Pseudomonas sp. strain PAMC 25931 was characterized as eurypsychrophilic (both psychrophilic and mesotolerant) with a broad temperature range of 5–30 °C both for anthranilate (2‐aminobenzoate) degradation and concomitant cell growth. Two degradative gene clusters (antABC and catBCA) were detected from a fosmid clone in the PAMC 25931 genomic library; each cluster was confirmed to be specifically induced by anthranilate. When expressed in Escherichia coli, the recombinant AntABC (anthranilate 1,2‐dioxygenase, AntDO) converted anthranilate into catechol, exhibiting strict specificity toward anthranilate. Recombinant CatA (catechol 1,2‐dioxygenase, C12O) from the organism was active over a broad temperature range (5–37 °C). However, CatA rapidly lost t...

  • Modelling during an emergency: the 2009 h1n1 influenza pandemic

    Updated: 2013-05-29 04:00:00
    This article is protected by copyright. All rights reserved. (Source: Clinical Microbiology and Infection)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Assessment of the environmental microbiological cross contamination following hand drying with paper hand towels or an air blade dryer

    Updated: 2013-05-29 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Applied Microbiology Journal of Applied Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues Virtual Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Virtual Issue on Biodefence Virtual Issue on Campylobacter Virtual

  • The C‐terminal Linker of Escherichia coli FtsZ Functions as an Intrinsically Disordered Peptide

    Updated: 2013-05-29 04:00:00
    The tubulin homolog FtsZ provides the cytoskeletal framework and constriction force for bacterial cell division. FtsZ has an ∼50 amino acid (aa) linker between the protofilament‐forming globular domain and the C‐terminal (Ct) peptide that binds FtsA and ZipA, tethering FtsZ to the membrane. This Ct linker is widely divergent across bacterial species and thought to be an intrinsically disordered peptide (IDP). We confirmed that the Ct linkers from three bacterial species behaved as IDPs in vitro by circular dichroism and trypsin proteolysis. We made chimeras, swapping the Escherichia coli linker for Ct linkers from other bacteria, and even for an unrelated IDP from human α‐adducin. Most substitutions allowed for normal cell division, suggesting that sequence of the IDP did not matt...

  • Acute inactivation of the Aspergillus nidulans Golgi membrane fusion machinery: correlation of apical extension arrest and tip swelling with cisternal disorganization

    Updated: 2013-05-29 04:00:00
    Summary The mechanisms governing traffic across the Golgi are incompletely understood. We studied, by live‐cell microscopy, the consequences of disorganizing the A. nidulans Golgi, using an extended set of fluorescent protein markers to resolve early from late cisternae. The early Golgi syntaxin SedVSed5 and the RabORab1 regulatory GTPase play essential roles in secretion, cooperating in the ER‐Golgi interface. Following a temperature shift‐up ‘on‐the‐stage’, hyphae carrying engineered sedVR258G and rabOA136D ts mutations arrest polarized growth. This arrest correlates with overall Golgi disorganization and characteristic hyphal tip swelling. Using v‐SNARE SynA as reporter, we show that the sedVR258G phenotypes correlate with arrested secretion. Both the morphogenetic defec...

  • Bypassing the need for subcellular localization of a polysaccharide export‐anchor complex by overexpressing its protein subunits

    Updated: 2013-05-29 04:00:00
    Summary Subcellular protein localization is thought to promote protein‐protein interaction by increasing the effective concentration and enabling spatial coordination and proper segregation of proteins. We found that protein overexpression allowed the assembly of a productive polysaccharide biosynthesis‐export‐anchoring complex in the absence of polar localization in Caulobacter crescentus. Polar localization of the holdfast export protein, HfsD, depends on the presence of the other export proteins, HfsA, and HfsB, and on the polar scaffold protein PodJ. The holdfast deficiency of hfsB and podJ mutants is suppressed by the overexpression of export proteins. Restored holdfasts are randomly positioned and co‐localize with a holdfast anchor protein in these strains, indicating that fu...

  • Saccharomyces cerevisiae deletion strains with complex DNA content profiles

    Updated: 2013-05-29 04:00:00
    This article is protected by copyright. All rights reserved. (Source: FEMS Microbiology Letters)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Enhancing beta‐carotene production in Saccharomyces cerevisiae by metabolic engineering

    Updated: 2013-05-29 04:00:00
    This article is protected by copyright. All rights reserved. (Source: FEMS Microbiology Letters)

  • Gingipain‐dependent degradation of mammalian target of rapamycin pathway proteins by the periodontal pathogen Porphyromonas gingivalis during invasion

    Updated: 2013-05-29 04:00:00
    In this study, we set out to investigate whether P. gingivalis and T. forsythia directly target key signalling molecules that may modulate the host cell phenotype to favour invasion and persistence. Our data identify, for the first time, that P. gingivalis, but not T. forsythia, reduces levels of intracellular mammalian target of rapamycin (mTOR) in oral epithelial cells following invasion over a 4‐h time course, via the action of gingipains. The ability of cytochalasin D to abrogate P. gingivalis‐mediated mTOR degradation suggests that this effect is dependent upon cellular invasion. We also show that levels of several other proteins in the mTOR signalling pathway are modulated by gingipains, either directly or as a consequence of mTOR degradation including p‐4E‐BP1. Taken to...

  • Cetrimonium Nalidixate as a Multifunctional Inhibitor to Combat Biofilm Formation and Microbiologically Influenced Corrosion

    Updated: 2013-05-29 04:00:00
    Marianne Seter, Melanie J. Thomson, Alison Chong, Douglas R. MacFarlane, Maria Forsyth Cetrimonium nalidixate salt, when incorporated into a polyurethane coating, leads to complete inhibition of microbiologically influenced corrosion in the presence of several bacteria strains commonly found in marine environments. (Source: Australian Journal of Chemistry)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Future of microbial polyesters

    Updated: 2013-05-28 04:00:00
    Log on BioMed Central Journals Gateways 3.55 Search this journal BioMed Central for Go Advanced search Home Articles Authors Reviewers About this journal My Microbial Cell Factories Microbial Cell Factories Volume 12 Viewing options Abstract Provisional PDF 1.4MB Associated material PubMed record About this article Readers' comments Related literature Cited by Google blog search Other articles by authors on Google Scholar Lee GN Na J on PubMed Lee GN Na J Related articles pages on Google on Google Scholar on PubMed Tools Download references Email to a friend Order reprints Post a comment Share this article Tweet More options . Citeulike LinkedIn Del.icio.us Email Facebook Google+ Mendeley Twitter Commentary Future of microbial polyesters Gi Na Lee and Jonguk Na For all author emails ,

  • Diversity of Mycoplasma hominis clinical isolates from Bordeaux, France, as assessed by multiple-locus variable-number tandem repeat analysis

    Updated: 2013-05-28 04:00:00
    Conclusions: Our MLVA scheme highlights the high genetic heterogeneity of the M. hominis species. It seems too discriminatory to be used for large epidemiological studies but has proven its usefulness for molecular studies at the individual level. (Source: BMC Microbiology - Latest articles)

  • Production, partial purification and characterization of protease from a phytopathogenic fungi Alternaria solani (Ell. and Mart.) Sorauer

    Updated: 2013-05-28 04:00:00
    , . . Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Journal of Basic Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS Submit an Article OnlineOpen Author Guidelines ABOUT THIS JOURNAL Contact Editorial Board Advertise Overview SPECIAL FEATURES Cover Gallery Read Cover Story Wiley Job Network Research Paper Production , partial purification and characterization of protease

  • Simultaneous detection of gastrointestinal pathogens with a multiplex Luminex‐based molecular assay in stool samples from diarrhoeic patients

    Updated: 2013-05-28 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Infectious Disease Microbiology Clinical Microbiology and Infection Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Editor's Choice Wiley Job Network Original Article

  • Inadequate management of pregnancy‐associated listeriosis: lessons from four case‐reports

    Updated: 2013-05-28 04:00:00
    This article is protected by copyright. All rights reserved. (Source: Clinical Microbiology and Infection)

  • Impact of rapid microbial identification directly from positive blood cultures using Matrix‐Assisted Laser Desorption/Ionisation Time‐of‐Flight Mass Spectrometry (MALDI‐TOF MS) on patient management

    Updated: 2013-05-28 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Infectious Disease Microbiology Clinical Microbiology and Infection Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Editor's Choice Wiley Job Network Original

  • Determination of the mode of action of enterolysin A, produced by Enterococcus faecalis B9510

    Updated: 2013-05-28 04:00:00
    ConclusionsEnterolysin A cleaves the peptide bonds within the stem peptide as well as in the interpeptide bridge of Gram‐positive bacterial cell walls. This gives a possible reason for the broad spectrum of enterolysin A activity. Significance and Impact of the StudyThis is the first report identifying the cleavage site of enterolysin A within the cell walls of sensitive bacteria. This will help in identifying potential applications for enterolysin A. (Source: Journal of Applied Microbiology)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • CD134 is a cellular receptor for HHV-6B [Microbiology]

    Updated: 2013-05-28 04:00:00
    Human herpesvirus-6B (HHV-6B) is a T lymphotropic β-herpesvirus that is clearly distinct from human herpesvirus-6A (HHV-6A) according to molecular biological features. The International Committee on Taxonomy of Viruses recently classified HHV-6B as a separate species. The primary HHV-6B infection causes exanthem subitum and is sometimes associated with severe encephalopathy. More... (Source: Proceedings of the National Academy of Sciences)

  • Imaging of pathogen elimination by CD8+ T cells [Microbiology]

    Updated: 2013-05-28 04:00:00
    CD8+ T cells are specialized cells of the adaptive immune system capable of finding and eliminating pathogen-infected cells. To date it has not been possible to observe the destruction of any pathogen by CD8+ T cells in vivo. Here we demonstrate a technique for imaging the killing of liver-stage malaria... (Source: Proceedings of the National Academy of Sciences)

  • c-di-AMP receptor proteins [Microbiology]

    Updated: 2013-05-28 04:00:00
    Nucleotide signaling molecules are important messengers in key pathways that allow cellular responses to changing environments. Canonical secondary signaling molecules act through specific receptor proteins by direct binding to alter their activity. Cyclic diadenosine monophosphate (c-di-AMP) is an essential signaling molecule in bacteria that has only recently been discovered. Here... (Source: Proceedings of the National Academy of Sciences)

  • MLV glyco-Gag and capsid stability [Microbiology]

    Updated: 2013-05-28 04:00:00
    Pathogenic retroviruses have evolved multiple means for evading host restriction factors such as apolipoprotein B editing complex (APOBEC3) proteins. Here, we show that murine leukemia virus (MLV) has a unique means of counteracting APOBEC3 and other cytosolic sensors of viral nucleic acid. Using virus isolated from infected WT and APOBEC3... (Source: Proceedings of the National Academy of Sciences)

  • Human to mosquito transmission of dengue viruses [Microbiology]

    Updated: 2013-05-28 04:00:00
    Dengue is the most prevalent arboviral disease of humans. The host and virus variables associated with dengue virus (DENV) transmission from symptomatic dengue cases (n = 208) to Aedes aegypti mosquitoes during 407 independent exposure events was defined. The 50% mosquito infectious dose for each of DENV-1–4 ranged from 6.29... (Source: Proceedings of the National Academy of Sciences)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Akkermansia muciniphila controls host metabolism [Microbiology]

    Updated: 2013-05-28 04:00:00
    Obesity and type 2 diabetes are characterized by altered gut microbiota, inflammation, and gut barrier disruption. Microbial composition and the mechanisms of interaction with the host that affect gut barrier function during obesity and type 2 diabetes have not been elucidated. We recently isolated Akkermansia muciniphila, which is a mucin-degrading... (Source: Proceedings of the National Academy of Sciences)

  • Polar localization of FtsA/FtsZ in Agrobacterium [Microbiology]

    Updated: 2013-05-28 04:00:00
    Growth and cell division in rod-shaped bacteria have been primarily studied in species that grow predominantly by peptidoglycan (PG) synthesis along the length of the cell. Rhizobiales species, however, predominantly grow by PG synthesis at a single pole. Here we characterize the dynamic localization of several Agrobacterium tumefaciens components during... (Source: Proceedings of the National Academy of Sciences)

  • Leanyer orthobunyavirus nucleoprotein-RNA complex [Microbiology]

    Updated: 2013-05-28 04:00:00
    Negative-stranded RNA viruses cover their genome with nucleoprotein (N) to protect it from the human innate immune system. Abrogation of the function of N offers a unique opportunity to combat the spread of the viruses. Here, we describe a unique fold of N from Leanyer virus (LEAV, Orthobunyavirus genus, Bunyaviridae... (Source: Proceedings of the National Academy of Sciences)

  • Crystal structure of BUNV NP-RNA complex [Microbiology]

    Updated: 2013-05-28 04:00:00
    Bunyamwera virus (BUNV), which belongs to the genus Orthobunyavirus, is the prototypical virus of the Bunyaviridae family. Similar to other negative-sense single-stranded RNA viruses, bunyaviruses possess a nucleocapsid protein (NP) to facilitate genomic RNA encapsidation and virus replication. The structures of two NPs of members of different genera within the... (Source: Proceedings of the National Academy of Sciences)

  • Visualization of pinholin lesions in vivo [Microbiology]

    Updated: 2013-05-28 04:00:00
    Lambdoid phage 21 uses a pinholin–signal anchor release endolysin strategy to effect temporally regulated host lysis. In this strategy, the pinholin S2168 accumulates harmlessly in the bilayer until suddenly triggering to form lethal membrane lesions, consisting of S2168 heptamers with central pores (Source: Proceedings of the National Academy of Sciences)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Orthobunyavirus nucleoproteins [Microbiology]

    Updated: 2013-05-28 04:00:00
    All negative-sense, single-stranded RNA (−ssRNA) viruses encode a nucleoprotein (NP), the major function of which is to bind viral RNAs and encapsidate them as ribonucleoprotein complexes (RNPs) (1, 2). Consequently, the genomes of −ssRNA viruses do not exist as naked RNA but rather as protein–RNA complexes with high-order structures. In... (Source: Proceedings of the National Academy of Sciences)

  • Impact of gastrointestinal parasitic nematodes of sheep, and the role of advanced molecular tools for exploring epidemiology and drug resistance - an Australian perspective

    Updated: 2013-05-27 04:00:00
    In an Australian context, this article gives a concise account of the biology and epidemiology of the gastrointestinal nematodes (order Strongylida), and emphasizes the importance of using advanced molecular tools for specific diagnosis. It also gives a perspective on the possibility of harnessing genetic, genomic and bioinformatic technologies to better understand parasites and control parasitic diseases. Image: Gastrointestinal nematodes of sheep. (Source: Parasites and Vectors)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • An In Vitro Evaluation of Disinfection Protocols used for Needleless Connectors of Central Venous Catheters

    Updated: 2013-05-27 04:00:00
    This article is protected by copyright. All rights reserved. (Source: Letters in Applied Microbiology)

  • Random and Site‐Specific Mutagenesis of the Helicobacter pylori Ferric Uptake Regulator Provides Insight into Fur Structure‐Function Relationships

    Updated: 2013-05-27 04:00:00
    Summary The ferric uptake regulator (Fur) of Helicobacter pylori is a global regulator that is important for colonization and survival within the gastric mucosa. H. pylori Fur is unique in its ability to activate and repress gene expression in both the iron‐bound (Fe‐Fur) and apo forms (apo‐Fur). In the current study we combined random and site‐specific mutagenesis to identify amino acid residues important for both Fe‐Fur and apo‐Fur function. We identified 25 mutations that affected Fe‐Fur repression and 23 mutations that affected apo‐Fur repression, as determined by transcriptional analyses of the Fe‐Fur target gene amiE, and the apo‐Fur target gene, pfr. In addition, eight of these mutations also significantly affected levels of Fur in the cell. Based on regulatory p...

  • Structure of a Translocation Signal Domain Mediating Conjugative Transfer by Type IV Secretion Systems

    Updated: 2013-05-27 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Molecular Microbiology Molecular Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES 3D Interactive Images Faculty of 1000 Prizes Awarded by Molecular Microbiology Virtual Special Issues

  • Lyme disease spirochetes possess an aggrecan‐binding protease with aggrecanase activity

    Updated: 2013-05-27 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Molecular Microbiology Molecular Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES 3D Interactive Images Faculty of 1000 Prizes Awarded by Molecular Microbiology Virtual Special Issues

  • Target evaluation of the non‐coding csRNAs reveals a link of the two‐component regulatory system CiaRH to competence control in Streptococcus pneumoniae R6.

    Updated: 2013-05-27 04:00:00
    Summary The two‐component regulatory system CiaRH of Streptococcus pneumoniae controls 25 genes, five of which specify homologous small non‐coding csRNAs (cia‐dependent small RNAs). The csRNAs were predicted to act regulatory as base‐pairing sRNAs, but their targets have not been identified. By csRNA gene inactivations we established that the major phenotypes associated with a hyperactive CiaRH system, enhanced ß‐lactam resistance and prevention of genetic competence, are dependent on the csRNAs. Computational target predictions and evaluations by translational fusions identified six genes to be under csRNA control: spr0081, spr0371, spr0159, spr0551, spr1097, and spr2043(comC). Measuring the effect of single csRNAs on three targets indicated that they acted additively. One of t...

  • Role of uropathogenic Escherichia coli OmpT in the resistance against human cathelicidin LL‐37

    Updated: 2013-05-27 04:00:00
    This study aims to investigate the involvement of UPEC OmpT in LL‐37 degradation. An ompT deletion mutant was generated in the prototypical UPEC strain CFT073. Western blot analysis showed that the OmpT protein level is moderate in CFT073. In agreement, OmpT was shown to partially cleave LL‐37. However, no difference in the minimum inhibitory concentration of LL‐37 was observed between CFT073 and the ompT mutant. Plasmid complementation of ompT, which led to increased OmpT levels, resulted in complete cleavage of LL‐37 and a 4‐fold increase in the minimum inhibitory concentration. The analysis of other UPEC isolates showed similar OmpT activity levels as CFT073. Although UPEC OmpT can cleave LL‐37, we conclude that the low level of OmpT limits its contribution to LL‐37 resist...

  • Molecular diagnosis of psoas abscess secondary to Pott's spine

    Updated: 2013-05-26 21:18:06
    Tuberculosis (TB) is the world's leading infectious cause of mortality after acquired immunodeficiency syndrome (). The incidence of extrapulmonary and atypical manifestations of TB is increasing. In 10 to 25% of cases, TB presents as an extrapulmonary infection. The skeletal form of the disease is responsible for 3% of the total number of cases, with 50% of these being spinal TB, often called Pott's disease (). One of the complications of spinal TB is an abscess of the psoas muscle; however, reports of tuberculous psoas abscess (PA) remain rare. The exact incidence of PA is unknown, but it probably has increased in the last few years because of the availability of improved diagnostic tests. (Source: Clinical Microbiology Newsletter)

  • Silibinin inhibits hepatitis C virus entry into hepatocytes by hindering clathrin‐dependent trafficking

    Updated: 2013-05-26 21:16:04
    Summary Hepatitis C virus (HCV) is a global health concern infecting 170 million people worldwide. Previous studies indicate that the extract from milk thistle known as silymarin and its main component silibinin inhibit HCV infection. Here we investigated the mechanism of anti‐HCV action of silymarin‐derived compounds at the molecular level. By using live‐cell confocal imaging, single particle tracking, transmission electron microscopy and biochemical approaches on HCV‐infected human hepatoma cells and primary hepatocytes, we show that silibinin potently inhibits HCV infection and hinders HCV entry by slowing down trafficking through clathrin‐coated pits and vesicles. Detailed analyses revealed that silibinin altered the formation of both clathrin‐coated pits and vesicles in ce...

  • Isolation and characterization of Keratinibaculum paraultunense gen. nov., sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity

    Updated: 2013-05-26 21:15:43
    This article is protected by copyright. All rights reserved. (Source: FEMS Microbiology Letters)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Investigation of the methanogen‐related archaeal population structure in shallow sediments of the Pearl River Estuary, Southern China

    Updated: 2013-05-26 04:00:00
    This study is a significant step toward understanding methane cycling association of representative archaea in estuarine environments. (Source: Journal of Basic Microbiology)

  • Influence of sclerotia formation on ligninolytic enzyme production in Morchella crassipes

    Updated: 2013-05-26 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Journal of Basic Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS Submit an Article OnlineOpen Author Guidelines ABOUT THIS JOURNAL Contact Editorial Board Advertise Overview SPECIAL FEATURES Cover Gallery Read Cover Story Wiley Job Network Research Paper Influence of sclerotia formation on ligninolytic enzyme production in

  • Potential of the salt‐tolerant laccase‐producing strain Trichoderma viride Pers. NFCCI‐2745 from an estuary in the bioremediation of phenol‐polluted environments

    Updated: 2013-05-26 04:00:00
    . Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Journal of Basic Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS Submit an Article OnlineOpen Author Guidelines ABOUT THIS JOURNAL Contact Editorial Board Advertise Overview SPECIAL FEATURES Cover Gallery Read Cover Story Wiley Job Network Research Paper Potential of the salt-tolerant laccase-producing strain Trichoderma

  • Molecular characterization and lipase profiling of the yeasts isolated from environments contaminated with petroleum

    Updated: 2013-05-26 04:00:00
    This study is the first report showed lipase production by these species. The other lipase producing strains identified as Candida parapsilosis (D3), Rhodotorula muciloginosa (D17 and D42), Cryptococcus albidus (D24, D27, D30, and D56), and Wickerhamomyces anomalus (D46). (Source: Journal of Basic Microbiology)

  • Inhibition by EGTA of the formation of a biofilm by clinical strains of Staphylococcus aureus

    Updated: 2013-05-26 04:00:00
    The effect of EGTA on the adhesion and on the formation of a biofilm by two reference and eight clinical strains of Staphylococcus aureus was studied. All the clinical strains were isolated from patients from Kinshasa. Spa typing confirmed that these clinical strains were distinct. The Biofilm Ring Test (BFRT®) showed that EGTA (100 µM–10 mM) inhibited the adhesion of the four clinical methicillin‐resistant (MRSA) strains and the crystal violet staining method that it inhibited the formation of a biofilm by all the strains. Divalent cations abolished the effect of EGTA on the formation of a biofilm, specially in the clinical MRSA strains. EGTA had no effect on established biofilms. Only concentrations of EGTA higher than 10 mM were toxic to eukaryotic cells. Our results establi...

  • Identification and functional characteristics of chlorpyrifos‐degrading and plant growth promoting bacterium Acinetobacter calcoaceticus

    Updated: 2013-05-26 04:00:00
    A bacterial strain D10 with strong ability of degrading chlorpyrifos was isolated from rhizosphere of chives contaminated with pesticide. It was found that it's capable of utilizing chlorpyrifos as the sole source of carbon for growth, and within the first 4 days the extent of degradation at initial concentration of 100 mg L−1 was 60.0%. It also showed a high ability of degrading chlorpyrifos in sterilized soil, and the degradation reached up to 60.2% after 18 days. In addition, the strain D10 also showed multiple plant growth‐promoting traits of phosphate solubilization, indole‐3‐acetic acid and siderophore production. The results indicate that the strain D10 has potential in the application of pesticide‐degrading and plant growth promotion. Strain D10 was identified as Acin...

  • SecA2 is not required for secretion of the surface autolysin IspC in Listeria monocytogenes serotype 4b

    Updated: 2013-05-26 04:00:00
    Listeria monocytogenes is one of several Gram‐positive bacteria known to contain an auxiliary ATPase (SecA2) involved in the Sec secretion of a subset of proteins important to bacterial pathogenesis, including autolysins. It is not known if IspC, a novel surface‐associated autolysin essential for full virulence of L. monocytogenes serotype 4b, is SecA2‐dependent for secretion. By creating a secA2 gene deletion (ΔsecA2) mutant from the wild type (WT) L. monocytogenes serotype 4b strain, in combination with the proteomic analysis of surface proteins and those secreted into the medium from both the mutant and the WT, we confirmed previous findings that two autolysins (p60 and NamA) are SecA2‐dependent for secretion. However, this approach did not identify IspC as one of the surface p...

  • Molecular cloning and functional characterization of MnSOD from Dunaliella salina

    Updated: 2013-05-26 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Journal of Basic Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS Submit an Article OnlineOpen Author Guidelines ABOUT THIS JOURNAL Contact Editorial Board Advertise Overview SPECIAL FEATURES Cover Gallery Read Cover Story Wiley Job Network Research Paper Molecular cloning and functional characterization of Mn SOD from

  • Adaptation to tert‐butyl hydroperoxide at a plasma membrane level in the fission yeast Schizosaccharomyces pombe parental strain and its t‐BuOOH‐resistant mutant

    Updated: 2013-05-26 04:00:00
    The one‐gene mutant hyd1‐190 of the fission yeast Schizosaccharomyces pombe displayed four‐fold resistance to tert‐butyl hydroperoxide (t‐BuOOH) in comparison with its parental strain hyd+. The cells of hyd1‐190 exhibited a quantitative alteration in the sterol content and hence in the fatty acid composition of the plasma membrane, reflected in a two‐fold amphotericin B sensitivity, increased rigidity of the plasma membrane, revealed by an elevated (Δ7.9 °C) phase‐transition temperature, measured by means of electron paramagnetic resonance spectroscopy, and a significantly decreased uptake of glycerol. Treatment of the strains with a subinhibitory concentration (0.2 mM) of t‐BuOOH induced adaptation via modification of the sterol and fatty acid compositions, resulti...

  • Outer membrane proteins of Fusobacterium necrophorum subsp. necrophorum and subsp. funduliforme

    Updated: 2013-05-26 04:00:00
    . . Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Journal of Basic Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS Submit an Article OnlineOpen Author Guidelines ABOUT THIS JOURNAL Contact Editorial Board Advertise Overview SPECIAL FEATURES Cover Gallery Read Cover Story Wiley Job Network Research Paper Outer membrane proteins of Fusobacterium necrophorum subsp .

  • Halotolerant, acid‐alkali stable, chelator resistant and raw starch digesting α‐amylase from a marine bacterium Bacillus subtilis S8–18

    Updated: 2013-05-26 04:00:00
    A halotolerant α‐amylase having the ability of digesting the insoluble raw starches was characterized from Bacillus subtilis S8–18, a marine sediment isolate from Palk Bay region. The electrophoresis techniques unveiled that the α‐amylase was indeed a monomer with a molecular weight of 57 kDa. The optimum temperature and pH for the enzyme activity were 60 °C and 6.0 respectively. The enzyme was highly stable for 24 h over a wide range of pH from 4.0 to 12.0 by showing 84–94% activity. Interestingly, by retaining 72% activity even after 24 h, the enzyme also showed tolerance towards 28% NaCl. The α‐amylase retained a minimum of 93% residual activity in 1 mM concentration for the selected divalent metal ions. The enzyme was found to be chelator resistant as it remaine...

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