• Instrument restarts booting during programed sample running

    Updated: 2012-12-30 23:36:40
    Dear friends, I have a problem since one weaks the instrument sarts rebooting can say restrts during programed samole running. if some one have any experience help me .

  • Effect of Signal Peptides on the Secretion of β-Cyclodextrin Glucanotransferase in Lactococcus lactis NZ9000

    Updated: 2012-12-28 22:00:00
    J Mol Microbiol Biotechnol 2012;22:361–372 (DOI:10.1159/000343921) (Source: Journal of Molecular Microbiology and Biotechnology)

  • Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis

    Updated: 2012-12-28 06:16:11
    ConclusionsOur results indicate that the FilmArray® Biothreat Panel is a sensitive and selective assay for detecting the genetic signatures of Ba, Ft and Yp.© 2012 No claim to US Government works (Source: Journal of Applied Microbiology)

  • Rapid and sensitive detection of Curvularia lunata associated with maize leaf spot based on its Clg2p gene using semi‐nested PCR

    Updated: 2012-12-28 06:15:57
    ConclusionsThe proposed PCR‐based assay may be used for diagnosing and monitoring maize Curvularia leaf spot. Significance and Impact of the StudyThe semi‐nested PCR assay may provide researchers and laboratory technologists a tool to rapidly detect C. lunata, which causes maize Curvularia leaf spot, compared with histological examination.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology)

  • Phosphorylation of the Streptococcus pneumoniae Cell Wall Biosynthesis Enzyme MurC by a Eukaryotic‐like Ser/Thr Kinase

    Updated: 2012-12-28 06:15:32
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology FEMS Microbiology Letters Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues Virtual Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES FEMS Most Cited Articles FEMS Most Read Articles Virtual Issue :

  • What do we know about the yeast strains from the Brazilian fuel ethanol industry?

    Updated: 2012-12-28 04:00:00
    . . : Warning The NCBI web site requires JavaScript to function . more . NCBI Skip to main content Skip to navigation Resources All Resources Chemicals Bioassays BioSystems PubChem BioAssay PubChem Compound PubChem Structure Search PubChem Substance All Chemicals Bioassays Resources . DNA RNA BLAST Basic Local Alignment Search Tool BLAST Stand-alone E-Utilities GenBank GenBank : BankIt GenBank : Sequin GenBank : tbl2asn Genome Workbench Influenza Virus Nucleotide Database PopSet Primer-BLAST ProSplign Reference Sequence RefSeq RefSeqGene Sequence Read Archive SRA Splign Trace Archive UniGene UniSTS All DNA RNA Resources . Data Software BLAST Basic Local Alignment Search Tool BLAST Stand-alone Cn3D Conserved Domain Search Service CD Search E-Utilities GenBank : BankIt GenBank : Sequin

  • Radiation-resistant extremophiles and their potential in biotechnology and therapeutics.

    Updated: 2012-12-28 04:00:00
    This article aims to review the strategies by which microorganisms thrive in extreme radiation environments and discuss their potential uses in biotechnology and the therapeutic industry. The major challenges that lie ahead are also discussed. PMID: 23271672 [PubMed - as supplied by publisher] (Source: Applied Microbiology and Biotechnology)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Molecular investigation on carbapenems resistance among multidrug‐resistant Pseudomonas aeruginosa clinical isolated in Thailand

    Updated: 2012-12-28 04:00:00
    Abstract Carbapenems resistance among multidrug resistant (CR‐MDR) P. aeruginosa were isolated from tertiary hospitals in Thailand. Decreased expression of oprD mRNA (93.65%) was predominant followed by increased expression of mexAB‐oprM mRNA (92.06%) and mexXY mRNA (63.49%). Interestingly, there were 23 out of 126 (18.25%) isolates which were susceptible to imipenem with down‐regulated oprD expression and non‐up‐regulated mexCD‐oprJ mRNA expression. Metallo‐β‐lactamases production was clearly positive in 24 isolates (18.46%) and weakly positive in 12 isolates (9.23%). Among both of them, imp‐1, imp‐14, and vim‐2 were identified. Hyperproduction of AmpC β‐lactamase was the lowest prevalence rate (3.97%). It could be concluded that CR‐MDR P. aeruginosa clinical i...

  • Anaplasma phagocytophilum, Interferon gamma production and Stat1 signaling

    Updated: 2012-12-28 04:00:00
    In this study, we examined the role of activation of signal transducer and activator of transcription (Stat) 1 phosphorylation with A. phagocytophilum infection, and found it to be markedly increased at day 7 post infection compared to mock‐infected controls. This increase in phosphorylated Stat1 (pStat1) was significantly correlated with IFN‐γ production and inflammatory tissue injury. Since pStat1 operates as a transcription factor central to the generation of effectors of inflammatory injury, these data suggest that Stat1 signaling is involved in IFN‐γ‐mediated immunopathologic lesions and disease in A. phagocytophilum infection and could be an important target for intervention of disease. (Source: Microbiology and Immunology)

  • Accumulation of MHC class II+ CD11c− non‐lymphoid cells in the spleen during infection with Plasmodium yoelii is lymphocyte‐dependent

    Updated: 2012-12-28 04:00:00
    This study revealed a novel interaction between MHC II+CD11c− non‐lymphoid cells and lymphoid cells in the accumulation of these non‐lymphoid cells in the spleen during infection with P. yoelii. (Source: Microbiology and Immunology)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Genomic polymorphisms in 3β‐hydroxysterol Δ24‐reductase promoter sequences

    Updated: 2012-12-28 04:00:00
    Abstract We recently reported that 3β‐hydroxysterol Δ24‐reductase (DHCR24) is induced by hepatitis C virus (HCV) infection. In addition, upregulation of DHCR24 impaired p53 activity. In human hepatoma HuH‐7 cells, level of DHCR24 expression was higher than normal hepatic cell lines (WRL68) at transcriptional level. The genomic promoter sequence of DHCR24 was characterized and nucleotide substitution were observed in HuH‐7 cells at nucleotide number –1453; G to A, –1420, G to T, –488, A to C, and –200 G to C. The mutations of these sequences from HuH‐7 cell types to WRL68 cell types suppressed DHCR24 gene promoter activity. We further characterized the sequences in hepatocytes from patient tissues. Four HCV positive patient tissues with cirrhosis or HCC (#1, 2, 3, 5) pos...

  • HLA‐DRB1 and HLA–DQB1 genes and allergic broncopulmonary aspergillosis susceptibility and protection in patients with cystic fibrosis

    Updated: 2012-12-28 04:00:00
    Abstract Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity lung disease that affects patients with cystic fibrosis (CF) and asthmatic patients. Indeed, HLA‐DRB1 alleles have previously been associated with ABPA‐CF susceptibility although HLA‐DQB1 allele associations were not clearly established. Our aim was to study HLA class II associations in our ABPA‐CF patients and determine their role in susceptibility or protection. Patients with ABPA‐CF, CF patients without ABPA, asthmatic patients without ABPA (AST) and healthy controls were included in this study. DNA was extracted by automatic extractor. HLA‐DRB1 and ‐DQB1 genotyping was performed by luminex PCR‐SSO method (OneLambda, CA). Allele specific PCR‐SSP was also performed by high resolution analysis ...

  • Interleukin‐8 and CXCL10 expressions in oral keratinocytes and fibroblasts via Toll‐like receptors

    Updated: 2012-12-28 04:00:00
    Abstract Oral keratinocytes and fibroblasts may be the first line of host defense against oral microorganisms. Here, we tested the hypothesis that oral keratinocytes and fibroblasts recognize microbial components via Toll‐like receptors (TLRs) and participate in development of oral inflammation. Our results showed that immortalized oral keratinocytes (RT7), fibroblasts (GT1) and primary cells expressed mRNA of TLRs 1‐10. Interleukin‐8 (IL‐8) production from RT7 cells was induced by treatment with TLR1‐9 except for TLR7 agonist, while, GT1 cells were induced to produce IL‐8 by all TLR agonists tested except for TLR7 and TLR9. GT1 cells showed increased CXCL10 production following treatment with agonists for TLR1/2, TLR3, TLR4 and TLR5, while only those for only TLR3 and TLR5 inc...

  • Modeling amyloids in bacteria

    Updated: 2012-12-28 04:00:00
    An increasing number of proteins are being shown to assemble into amyloid structures, self-seeding fibrillar aggregates that may lead to pathological states or play essential biological functions in organisms. Bacterial cell factories have raised as privileged model systems to understand the mechanisms behind amyloid assembly and the cellular fitness cost associated to the formation of these aggregates. In the near future, these bacterial systems will allow implementing high-throughput screening approaches to identify effective modulators of amyloid aggregation. (Source: Microbial Cell Factories)

  • Whole genome HBV deletion profiles and the accumulation of preS deletion mutant during antiviral treatment

    Updated: 2012-12-28 04:00:00
    Conclusions: We present the HBV deletion distribution patterns and preS deletion substructures in viral genomes that are prevalent in northern China. The accumulation of preS deletion mutants during nucleos(t)ide analog therapy may be due to viral escape from host immuno-surveillance. (Source: BMC Microbiology - Latest articles)

  • Ca2+‐mediated exocytosis of subtilisin‐like protease 1: a key step in egress of Plasmodium falciparum merozoites

    Updated: 2012-12-28 04:00:00
    Summary Egress of Plasmodium falciparum merozoites from host erythrocytes is a critical step in multiplication of blood‐stage parasites. A cascade of proteolytic events plays a major role in degradation of membranes leading to egress of merozoites. However, the signals that regulate the temporal activation and/or secretion of proteases upon maturation of merozoites in intra‐erythrocytic schizonts remain unclear. Here, we have tested the role of intracellular Ca2+ in regulation of egress of P. falciparum merozoites from schizonts. A sharp rise in intracellular Ca2+ just before egress, observed by time‐lapse video microscopy, suggested a role for intracellular Ca2+ in this process. Chelation of intracellular Ca2+ with chelators such as BAPTA‐AM or inhibition of Ca2+ release from in...

  • Statistical analyses of protein sequence alignments identify structures and mechanisms in signal activation of sensor histidine kinases

    Updated: 2012-12-28 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Molecular Microbiology Molecular Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES 3D Interactive Images Faculty of 1000 Prizes Awarded by Molecular Microbiology Virtual Special Issues Wiley Job

  • Galactomannan Biosynthesis [Microbiology]

    Updated: 2012-12-28 04:00:00
    Skip to main page content Home Current issue Archive Papers in Press Minireviews Reports Classics Reflections Papers of the Week QUICK SEARCH : Author : Keyword : Year : Vol : Page GO Advanced Search Browse the Archive Advertisement Advertisement Biosynthesis of the Fungal Cell Wall Polysaccharide Galactomannan Requires Intraluminal GDP-mannose Jakob Engel 1 Philipp S . Schmalhorst 1 2 and Françoise H . Routier 3 From the Department of Cellular Chemistry , OE 4330, Hannover Medical School , Carl-Neuberg-Strasse 1, 30625 Hannover , Germany 3 To whom correspondence should be addressed . Tel . 49-511-5329807 Fax : 49-511-5328801 E-mail : Routier.Francoise{at mh-hannover.de 1 Both authors contributed equally to this work . : Background Understanding the mechanisms of cell wall biogenesis is

  • Membrane Interactions of ESAT-6 from M. tuberculosis and M. smegmatis [Molecular Bases of Disease]

    Updated: 2012-12-28 04:00:00
    In conclusion, we have demonstrated that MtbESAT-6 possesses a unique membrane-interacting activity that is not found in MsESAT-6 and established the utility of rigorous biochemical approaches in dissecting the virulence of M. tuberculosis. (Source: Journal of Biological Chemistry)

  • Biocatalytic potential of laccase-like multicopper oxidases from Aspergillus niger

    Updated: 2012-12-27 04:00:00
    Conclusions: The A. niger McoA, McoB, and McoG enzymes showed clearly different catalytic properties. Yellow McoB showed broad substrate specificity, catalyzing the oxidation of several phenolic compounds commonly present in different industrial effluents. It also harbored high decolorization and detoxification activity with the synthetic dye malachite green, showing to have an interesting potential as a new industrial biocatalyst. (Source: Microbial Cell Factories)

  • Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)

    Updated: 2012-12-27 04:00:00
    Conclusions: The VIDISCR method was able to identify known virus isolates and a novel isolate of Getah virus from infected mice. Phylogenetic analysis indicated that the YN08 isolate was more closely related to the Hebei HB0234 strain than the YN0540 strain, and more genetically distinct from the MM2021 Malaysia primitive strain. (Source: BMC Microbiology - Latest articles)

  • PyroTRF-ID: a novel bioinformatics methodology for the affiliation of terminal-restriction fragments using 16S rRNA gene pyrosequencing data

    Updated: 2012-12-27 04:00:00
    : Log on BioMed Central Journals Gateways 3.04 Search BMC Microbiology BioMed Central for Go Advanced search Home Articles Authors Reviewers About this journal My BMC Microbiology BMC Microbiology Volume 12 Viewing options Abstract Provisional PDF 317KB Associated material PubMed record About this article Readers' comments Related literature Cited by on Google blog search Other articles by authors on Google Scholar Weissbrodt DG Shani N Sinclair L Lefebvre G Rossi P Maillard J Rougemont J Holliger C on PubMed Weissbrodt DG Shani N Sinclair L Lefebvre G Rossi P Maillard J Rougemont J Holliger C Related articles pages on Google on Google Scholar on PubMed Tools Download references Email to a friend Order reprints Post a comment Share this article Tweet More options . Citeulike Connotea

  • Cloning, expression and decoding of the cold adaptation of a new widely represented thermolabile subtilisin‐like protease

    Updated: 2012-12-27 04:00:00
    ConclusionsA new thermolabile subtilisin‐like protease has been successfully cloned and analysed, and an important hot spot in the evolution of the cold adaptation and substrate specificity of this enzyme was identified and tested. Significance and Impact of the StudyThis work reports a new cold‐adapted protease with a vast representation amongst Antarctic genus, suggesting therefore its evolutionary success in this cold environment. Likewise, important sites for genetic potentiation have been identified, which are extrapolated to other enzymes of the same kind. (Source: Journal of Applied Microbiology)

  • Responses of community structure of amoA‐encoding archaea and ammonia‐oxidizing bacteria in ammonia biofilter with rockwool mixtures to the gradual increases in ammonium and nitrate

    Updated: 2012-12-27 04:00:00
    ConclusionsThe specific FA in circulation water seems to be the important factor, which relates to the AOB and AEA community shifts in the biofilter besides ammonium and pH. Significance and Impact of the StudyOne of the key factors for regulating AEA and AOB communities was proposed that is useful for optimizing biofiltration technology. (Source: Journal of Applied Microbiology)

  • Recovery balance: a method for estimating losses in a Bacillus anthracis spore sampling protocol

    Updated: 2012-12-27 04:00:00
    ConclusionsThe approach used in this study pointed out the need for a closer analysis of the complex interaction between spores and wipe material because a substantial percentage of spores were lost to the wipe. Significance and Impact of the StudyThe recovery balance, in association with independent controls, provides an account for error contribution and potential variability on each step of the sampling protocol. The approach is not meant to be a replacement for field or laboratory validation of wipe recoveries but promote the development of new collection methodologies and support protocol optimization in laboratory settings. (Source: Journal of Applied Microbiology)

  • Study of psychrophilic and psychrotolerant micro‐organisms isolated in cold rooms used for pharmaceutical processing

    Updated: 2012-12-27 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Applied Microbiology Journal of Applied Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues Virtual Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Virtual Issue on Biodefence Virtual Issue on Campylobacter Virtual

  • Two new Penicillium species Penicillium buchwaldii and Penicillium spathulatum, producing the anticancer compound asperphenamate

    Updated: 2012-12-27 04:00:00
    , Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology FEMS Microbiology Letters Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues Virtual Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES FEMS Most Cited Articles FEMS Most Read Articles Virtual Issue :

  • Protective effect of mannanoligossacharides against early colonization by Salmonella Enteritidis in chicks is improved by higher dietary threonine levels

    Updated: 2012-12-26 04:00:00
    ConclusionMannanoligossacharides and threonine act synergistically, resulting in improved intestinal environment and recovery after Salmonella inoculation. Significance and impact of studyNutritional approaches may be useful to prevent Salmonella infection in the first week and putative carcass contamination at slaughter. This is the first report on the possible synergistic effect of mannanoligosaccharides and threonine, and further studies should be performed including performance, microbiota evaluation, composition of intestinal mucins and immune assessment.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Presence of enterotoxigenic Escherichia coli in biofilms formed in water containers in poor households coincides with epidemic seasons in Dhaka

    Updated: 2012-12-26 04:00:00
    ConclusionsWe conclude that ETEC is continuously present in water and biofilms in household water reservoirs in Dhaka which has a high prevalence of ETEC diarrhea. The frequency of biofilms with ETEC was significantly associated (p < 0.008) to seasonal epidemic peaks of ETEC diarrhea. Significance and impact of the studyWe show for the first time that enterotoxigenic Escherichia coli (ETEC), the causative agent of acute watery diarrhea and travelers’ diarrhea is present in biofilms in household water tanks in Dhaka, Bangladesh.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Utilization and optimization of a waste stream cellulose culture medium for pigment production by Penicillium spp

    Updated: 2012-12-26 04:00:00
    ConclusionsP. resticulosum demonstrated microbial pH‐stable‐pigment production potential using a xylose or CMC‐, and N‐source, supplemented waste stream cellulose culture medium. Significance and Impact of StudyCorn derived, waste stream cellulose can be used as a culture medium for fungal pigment production. Such application provides a process for agricultural waste stream resource reuse for production of compounds in increasing demand.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Evaluation of Diffusion and Dilution Methods to Determine the Antimicrobial Activity of Water‐Soluble Chitosan Derivatives

    Updated: 2012-12-26 04:00:00
    ConclusionsBesides chitosan's molecular weight and bacterial genus/species, the antimicrobial activity of chitosan was also influenced largely by the susceptibility testing method used. Significance and Impact of StudyThis is the first study that comparatively evaluated these diffusion and dilution methods, particularly two quantitative methods (agar dilution and broth microdilution), to assess the antimicrobial activity of two water‐soluble chitosans against a large number of foodborne pathogens. The study highlights the need for standardized methods to be used in evaluating chitosan's antimicrobial properties in future studies.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Isolation and characterization of two thermophilic cellulolytic strains of Clostridium thermocellum from a compost sample

    Updated: 2012-12-26 04:00:00
    ConclusionsCS7 and CS8 are two new highly cellulolytic and ethanologenic C. thermocellum strains. Significance and Impact of the StudyFirst report of applying the cloning‐RFLP‐sequencing approach for purity confirmation of the isolates beside conventional methods. Strains CS7 and CS8 might be of potential application in research and development of cellulosic bioconversion.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)<bMedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Shiga toxin‐producing Escherichia coli (STEC) in drinking water supplies of north Paraná State, Brazil

    Updated: 2012-12-26 04:00:00
    ConclusionDrinking water, especially from untreated water supplies, can be source of STEC strains potentially pathogenic for humans.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • The impact and mode of action of phenolic compounds extracted from brown seaweed on mixed anaerobic microbial cultures

    Updated: 2012-12-26 04:00:00
    ConclusionsResults suggest that the bactericidal activity of phlorotannins is a function of the level of polymerisation of the compounds. By monitoring intermediary compounds during the anaerobic digestion of phlorotannins, it was also found that higher energy consumption is required by microorganisms for survival under stress induced by phlorotannins.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Evaluating glucose and xylose as co‐substrates for lipid accumulation and γ‐linolenic acid biosynthesis of Thamnidium elegans

    Updated: 2012-12-26 04:00:00
    ConclusionsT. elegans represents a promising fungus for the successful valorization of sugar‐based lignocellulosic residues into microbial lipids of high nutritional and pharmaceutical interest.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Rapid enumeration of Oenococcus oeni during malolactic fermentation by flow cytometry

    Updated: 2012-12-26 04:00:00
    ConclusionFCM coupled with the BaclightTM kit makes it possible to count O. oeni cells during MLF with a perfect correlation with the plate count method.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Sporulation environment of emetic toxin‐producing Bacillus cereus strains determines spore size, heat resistance and germination capacity

    Updated: 2012-12-26 04:00:00
    ConclusionsSporulation in complex conditions such as biofilms and surface swarming colonies increases heat resistance and dormancy of spores.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Evaluation of a IS6110‐Taqman real‐ time PCR assay to detect Mycobacterium tuberculosis in sputum samples of patients with pulmonary TB

    Updated: 2012-12-26 04:00:00
    ConclusionsThe IS6110‐TaqMan was rapid, sensitive and specific for the diagnosis of pulmonary TB.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Effects of thermosonication on the fate of Escherichia coli O157:H7 and Salmonella Enteritidis in mango juice

    Updated: 2012-12-26 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Applied Microbiology Letters in Applied Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues Virtual Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Antimicrobials Virtual Issue Wiley Job Network Wine

  • Benzamidine derivatives inhibit the virulence of Porphyromonas gingivalis

    Updated: 2012-12-26 04:00:00
    Summary We have previously shown that benzamidine‐type compounds can inhibit the activity of arginine‐specific cysteine proteinases (gingipains HRgpA and RgpB); well‐known virulence factors of Porphyromonas gingivalis. They also hinder in vitro growth of this important periodontopathogenic bacterium. Apparently growth arrest is not associated with their ability to inhibit these proteases, because pentamidine, which is a 20‐fold less efficient inhibitor of gingipain than 2,6‐bis‐(4‐amidinobenzyl)‐cyclohexanone (ACH), blocked P. gingivalis growth far more effectively. To identify targets for benzamidine‐derived compounds other than Arg‐gingipains, and to explain their bacteriostatic effects, P. gingivalis ATCC 33277 and P. gingivalis M5‐1‐2 (clinical isolate) cell ...

  • Chest computed tomography predicts microbiological burden and symptoms in pulmonary Mycobacterium xenopi

    Updated: 2012-12-26 04:00:00
    Conclusions:  Among immunocompetent patients with pulmonary M. xenopi isolation, cavitation and large nodules predict fulfilling microbiological disease criteria, while bronchiectasis and small nodules predict symptoms. (Source: Respirology)

  • The physicochemical and microbiological quality of the Sudanese yoghurt Mish produced by traditional and modernised methods

    Updated: 2012-12-26 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Food Science Technology General Introductory Food Science Technology International Journal of Dairy Technology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Extended Shelf Life Milk Job Vacancies Wiley Job Network Original

  • Neisseria gonorrhoeae Breaches the Apical Junction of Polarized Epithelial Cells for Transmigration by activating EGFR

    Updated: 2012-12-26 04:00:00
    This study examines the interaction of gonococci (GC) with polarized epithelial cells. We show that viable GC preferentially localize at the apical side of the cell‐cell junction in polarized endometrial and colonic epithelial cells, HEC‐1‐B and T84. In GC infected cells, continuous apical junctional complexes are disrupted, and the junction‐associated protein β‐catenin is redistributed from the apical junction to the cytoplasm and to GC adherent sites, however overall cellular levels remain unchanged. This redistribution of junctional proteins is associated with a decrease in the ‘fence’ function of the apical junction but not its ‘gate’ function. Disruption of the apical junction by removing calcium increases GC transmigration across the epithelial monolayer. GC inocul...

  • Type III secretion translocon assemblies that attenuate Yersinia virulence

    Updated: 2012-12-26 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology Cellular Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Faculty of 1000 Parasitology Virtual Special Issue Posters Virology Virtual Special Issue Wiley Job Network

  • The Role of Galectin‐3 in Phagocytosis of Candida albicans and Candida parapsilosis by Human Neutrophils

    Updated: 2012-12-26 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology Cellular Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Faculty of 1000 Parasitology Virtual Special Issue Posters Virology Virtual Special Issue Wiley Job Network

  • The group A streptococcal collagen‐like protein‐1, Scl1, mediates biofilm formation by targeting the extra domain A‐containing variant of cellular fibronectin expressed in wounded tissue

    Updated: 2012-12-26 04:00:00
    Summary Wounds are known to serve as portals of entry for group A Streptococcus (GAS). Subsequent tissue colonization is mediated by interactions between GAS surface proteins and host extracellular matrix components. We recently reported that the streptococcal collagen‐like protein‐1, Scl1, selectively binds the cellular form of fibronectin (cFn) and also contributes to GAS biofilm formation on abiotic surfaces. One structural feature of cFn, which is predominantly expressed in response to tissue injury, is the presence of a spliced variant containing extra domain A (EDA/EIIIA). We now report that GAS biofilm formation is mediated by the Scl1 interaction with EDA‐containing cFn. Recombinant Scl1 proteins that bound cFn also bound recombinant EDA within the C‐C’ loop region recogn...

  • SPP inhibition as antiparasitic target [Microbiology]

    Updated: 2012-12-26 04:00:00
    Early secretory and endoplasmic reticulum (ER)-localized proteins that are terminally misfolded or misassembled are degraded by a ubiquitin- and proteasome-mediated process known as ER-associated degradation (ERAD). Protozoan pathogens, including the causative agents of malaria, toxoplasmosis, trypanosomiasis, and leishmaniasis, contain a minimal ERAD network relative to higher eukaryotic cells, and, because... (Source: Proceedings of the National Academy of Sciences)

  • Structural basis of toxicity and immunity in CDI [Microbiology]

    Updated: 2012-12-26 04:00:00
    We present crystal structures of CDI toxin/immunity complexes from Escherichia coli EC869 and Burkholderia pseudomallei 1026b. Despite sharing little sequence identity, the toxin domains are structurally similar and have homology to endonucleases. The EC869... (Source: Proceedings of the National Academy of Sciences)MedWorm Sponsor Message: Find the best Christmas presents and January Sales in the UK with this simple shopping directory.

  • Mutually exclusive expressions in P. falciparum [Microbiology]

    Updated: 2012-12-26 04:00:00
    Plasmodium falciparum causes the deadliest form of human malaria. Its virulence is attributed to its ability to modify the infected RBC and to evade human immune attack through antigenic variation. Antigenic variation is achieved through tight regulation of antigenic switches between variable surface antigens named “P. falciparum erythrocyte membrane protein-1”... (Source: Proceedings of the National Academy of Sciences)

  • Comparative lipidomics analysis of HIV‐1 particles and their producer cell membrane in different cell lines

    Updated: 2012-12-26 00:16:37
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology Cellular Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Faculty of 1000 Parasitology Virtual Special Issue Posters Virology Virtual Special Issue Wiley Job Network

  • The 2‐methylcitrate cycle is implicated in the detoxification of propionate in Toxoplasma gondii

    Updated: 2012-12-26 00:15:39
    Summary Toxoplasma gondii belongs to the coccidian‐subgroup of the Apicomplexa phylum. The Coccidia are obligate intracellular pathogens that establish infection in their mammalian host via the enteric route. These parasites lack a mitochondrial pyruvate dehydrogenase complex but have preserved the degradation of branched‐chain amino acids (BCAA) as a possible pathway to generate acetyl‐CoA. Importantly, degradation of leucine, isoleucine and valine could lead to concomitant accumulation of propionyl‐CoA, a toxic metabolite that inhibits cell growth. Like fungi and bacteria, the Coccidia possess the complete set of enzymes necessary to metabolize and detoxify propionate by oxidation to pyruvate via the 2‐methylcitrate cycle (2‐MCC). Phylogenetic analysis provides evidence that ...

  • HireLifeScience.com - Santa bringing #jobs? New this AM @ PRA Boehringer AstraZeneca GSK Novartis & MedImmune

    Updated: 2012-12-24 05:40:45
    Link to today's jobs: http://www.hirelifescience.com/seeker_jobs.aspx?q=1&keyword=Any%20job%20title%20or%20keyword Sartorius Group North America jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=126 Warner Chilcott jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=163 Boehringer-Ingelheim jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=106 PRA International jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=147 ...

  • Iron regulated surface determinant B (IsdB) promotes Staphylococcus aureus adherence to and internalization by non‐phagocytic human cells.

    Updated: 2012-12-24 04:00:00
    . Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology Cellular Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Faculty of 1000 Parasitology Virtual Special Issue Posters Virology Virtual Special Issue Wiley Job

  • The microtubule motor protein KIF13A is involved in intracellular trafficking of the Lassa virus matrix protein Z

    Updated: 2012-12-24 04:00:00
    Summary The small matrix protein Z of arenaviruses has been identified as the main driving force to promote viral particle production at the plasma membrane. Although multiple functions of Z in the arenaviral life cycle have been uncovered, the mechanism of intracellular transport of Z to the site of virus budding is poorly understood and cellular motor proteins that mediate Z trafficking remain to be identified. In the present study, we report that the Z protein of the Old World arenavirus Lassa virus (LASV) interacts with the kinesin family member 13A (KIF13A), a plus end‐directed microtubule‐dependent motor protein. Plasmid‐driven overexpression of KIF13A results in relocalization of Z to the cell periphery, while functional blockage of endogenous KIF13A by overexpression of a dom...

  • Cloning and expression of synthetic genes encoding ACE inhibitory bioactive peptides in Bifidobacterium pseudocatenulatum

    Updated: 2012-12-24 04:00:00
    Abstract A wide range of biopeptides potentially able to lower blood pressure through inhibition of the Angiotensin‐I Converting Enzyme are produced in fermented foods by proteolytic starter cultures. This work applies a procedure based on recombinant DNA technologies for the synthesis and expression of three ACE‐inhibitory peptides using a probiotic cell factory. ACE‐inhibitory genes and their pro‐active precursors were designed, synthesized by PCR, and cloned in Escherichia coli; after which, they were cloned into the pAM1 E. coli‐bifidobacteria shuttle vector. After E. coli transformation, constructs carrying the six recombinant clones were electrotransferred into the Bifidobacterium pseudocatenulatum M115 probiotic strain. Interestingly, five out of the six constructs proved ...

  • Enzymatic and genetic characterization of the DasD protein possessing N‐acetyl‐β‐D‐glucosaminidase activity in Streptomyces coelicolor A3(2)

    Updated: 2012-12-24 04:00:00
    Abstract The dasD gene is located just downstream of the dasABC gene cluster, encoding components of an ABC transporter for uptake of a chitin‐degradation product N,N’‐diacetylchitobiose [(GlcNAc)2] in Streptomyces coelicolor A3(2). To clarify the roles of the DasD protein in the degradation and assimilation of chitin, we obtained and characterized a recombinant DasD protein and a dasD‐null mutant of S. coelicolor A3(2). The recombinant DasD protein produced in Escherichia coli showed N‐acetyl‐β‐D‐glucosaminidase (GlcNAcase) activity and its optimum temperature and pH were 40°C and 7, respectively. dasD transcription was strongly induced in the presence of chitin, weakly by chitosan, but not by cellulose or xylan in S. coelicolor A3(2). Immuno‐blot analysis demonstrated...

  • Taguchi Optimization of Duplex PCR for Simultaneous Identification of Staphylococcus aureus and Clostridium perfringens Alpha Toxins

    Updated: 2012-12-24 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology FEMS Microbiology Letters Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues Virtual Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES FEMS Most Cited Articles FEMS Most Read Articles Virtual Issue :

  • HireLifeScience.com - #CareerUpgradeFriday @ PRA Boehringer AstraZeneca GSK DaiichiSankyo Agilent

    Updated: 2012-12-21 07:17:30
    Link to today's jobs: http://www.hirelifescience.com/seeker_jobs.aspx?q=1&keyword=Any%20job%20title%20or%20keyword Sartorius Group North America jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=126 Warner Chilcott jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=163 Boehringer-Ingelheim jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=106 PRA International jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=147 ...

  • Making changes for 2013? New #jobs @ PRA Boehringer GE AstraZeneca GSK Agilent Novartis MedImmune & WarnerChilcott

    Updated: 2012-12-19 06:38:14
    Link to today's jobs: http://www.hirelifescience.com/seeker_jobs.aspx?q=1&keyword=Any%20job%20title%20or%20keyword Sartorius Group North America jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=126 Warner Chilcott jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=163 Boehringer-Ingelheim jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=106 PRA International jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=147 ...

  • Career Upgrade Friday at HireLifeScience.com

    Updated: 2012-12-07 05:21:20
    CareerUpgradeFriday @ Sartorius, PRA, WarnerChilcott, MedImmune, Boehringer, GSK, AstraZeneca, Novartis & Neogen #pharma #biotech #device #jobs Link to today's jobs: http://www.hirelifescience.com/seeker_jobs.aspx?q=1&keyword=Any%20job%20title%20or%20keyword Sartorius Group North America jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=126 Warner Chilcott jobs: http://www.hirelifescience.com/companyjobs.aspx?CompanyId=163 Boehringer-Ingelheim jobs: http://...

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