• Eudragit E100® potentiates the bactericidal action of ofloxacin against fluoroquinolone‐resistant Pseudomonas aeruginosa

    Updated: 2012-06-22 21:45:39
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology FEMS Microbiology Letters Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES FEMS Most Cited Articles FEMS Most Read Articles Virtual Issue : Pathogenic and

  • Mechanism of Invasion of Lung Epithelial Cells by Filamentous Legionella pneumophila

    Updated: 2012-06-22 10:06:51
    SummaryLegionella, the etiological agent responsible for legionellosis is an opportunistic pathogen that infects humans upon the inhalation of contaminated aerosolized water droplets. Legionella is pleomorphic and its different morphotypes exhibit varying degrees of virulence. While the filamentous forms of Lp have been reported in patient samples since the first description of legionellosis, their role in disease has not been studied. Our results show that both E‐cadherin and β1 integrin receptors mediate FLp attachment to lung epithelial cells (LECs). The activation of these receptors induces the formation of actin enriched membrane surface structures that we designated ‘hooks’ and ‘membrane wraps’. These structures entrap the filaments on the cell surface leading to their gra...

  • Immunoglobulin domains in E. coli and other enterobacteria: from pathogenesis to applications in antibody technologies

    Updated: 2012-06-22 10:05:31
    AbstractThe immunoglobulin (Ig) protein domain is widespread in nature having a well‐recognized role in proteins of the immune system. In this review we describe the proteins containing Ig‐like domains in E. coli and enterobacteria, reporting their structural and functional properties, protein folding and diverse biological roles. In addition, we cover the expression of heterologous Ig domains in E. coli due to its biotechnological application for expression and selection of antibody fragments and full‐length IgG molecules. Ig‐like domains in E. coli and enterobacteria are frequently found in cell surface proteins and fimbrial organelles playing important functions during host cell adhesion and invasion of pathogenic strains, being structural components of pilus and non‐pilus fim...

  • Dual mechanism for Mycobacterium tuberculosis cytotoxicity on lung epithelial cells

    Updated: 2012-06-22 07:02:08
    Canadian Journal of Microbiology, Volume 0, Issue 0, Page 909-918, e-First articles. (Source: Canadian Journal of Microbiology)

  • The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis

    Updated: 2012-06-22 04:00:00
    Conclusions: The results demonstrate that MH cockroaches are an attractive alternative to mammals to study host-pathogen interactions and may allow the identification of new Burkholderia virulence determinants. The importance of T6SS-1 as a virulence factor in MH cockroaches and rodents suggests that the primary role of this secretion system is to target evasion of the innate immune system. (Source: BMC Microbiology - Latest articles)

  • Genetic diversity and molecular typing of Listeria monocytogenes in China

    Updated: 2012-06-22 04:00:00
    Conclusions: STs prevalent in other parts of the world are also prevalent in China including 7 STs (ST1-ST3, ST5, ST6, ST8, ST9) which caused maternal fetal infections or outbreaks, suggesting that these STs potentially can also cause severe human infections or outbreaks in China. Surveillance of these STs will provide important information for prevention of listeriosis. This study also enhances our understanding of genetic diversity of L. monocytogenes in China. (Source: BMC Microbiology - Latest articles)

  • Molecular analysis of meso- and thermophilic microbiota associated with anaerobic biowaste degradation

    Updated: 2012-06-22 04:00:00
    Conclusions: The fungal community survives in anoxic conditions and grows with increasing organic loading, suggesting that Fungi may contribute to the digestion by metabolising organic nutrients for bacterial and methanogenic groups. The microarray proof of principle tests suggest that the method has the potential for semiquantitative detection of target microbial groups given that comprehensive sequence data is available for probe design. (Source: BMC Microbiology - Latest articles)

  • Metagenome analyses of corroded concrete wastewater pipe biofilms reveals a complex microbial system

    Updated: 2012-06-22 04:00:00
    Conclusions: The functional potential of wastewater biofilms was highly diverse with level of COG diversity similar to that described for soil. On the basis of the metagenomic data, some factors that may contribute to niche differentiation were pH, aerobic conditions and availability of substrate, such as nitrogen and sulfur. The results from this study will help us better understand the genetic network and functional capability of microbial members of wastewater concrete biofilms. (Source: BMC Microbiology - Latest articles)MedWorm Sponsor Message: Please have a look at this new site driven by MedWorm: The Breast Cancer Daily

  • Sequential deletion of all the polyketide synthase and nonribosomal peptide synthetase biosynthetic gene clusters and a 900‐kb subtelomeric sequence of the linear chromosome of Streptomyces coelicolor

    Updated: 2012-06-22 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology FEMS Microbiology Letters Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES FEMS Most Cited Articles FEMS Most Read Articles Virtual Issue : Pathogenic and

  • Diversity of Methyl Halide‐Degrading Microorganisms in Oceanic and Coastal Waters

    Updated: 2012-06-22 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology FEMS Microbiology Letters Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES FEMS Most Cited Articles FEMS Most Read Articles Virtual Issue : Pathogenic and

  • Ornithine lipids and their structural modifications: from A to E and beyond

    Updated: 2012-06-22 04:00:00
    AbstractOrnithine lipids (OLs) are phosphorus‐free membrane lipids that are widespread in eubacteria, but absent from archaea and eukaryotes. They contain a 3‐hydroxy fatty acyl group attached in amide linkage to the α‐amino group of the amino acid ornithine. A second fatty acyl group is ester‐linked to the 3‐hydroxy position of the first fatty acid. About 25% of the bacterial species whose genomes have been sequenced are predicted to have the capacity to form OLs. Distinct OL hydroxylations have been described in the ester‐linked fatty acid, the amide‐linked fatty acid, and in the ornithine moiety. These modifications often seem to form part of a bacterial stress response to changing environmental conditions, allowing the bacteria to adjust membrane properties by simply mod...

  • Oxidative stress response in two representative bacteria exposed to atrazine

    Updated: 2012-06-22 04:00:00
    AbstractBacteria are present extensively in the environment. The investigation of their antioxidant properties will be useful for further study on atrazine stress tolerance of bacteria and defense mechanism of antioxidant enzymes against atrazine or other triazine herbicides. Superoxide dismutase (SOD), catalase (CAT), glutathione S‐transferase (GST) and total antioxidant capacity (T‐AOC), from one Gram‐negative representative strain Escherichia coli K12 and one Gram‐positive representative strain Bacillus subtilis B19, respectively, were tested for response to atrazine stress. The results indicated that SOD, CAT, GST activities and T‐AOC were induced upon exposure to atrazine. The growth of two bacteria in the absence of atrazine was better than in the presence of atrazine, indi...

  • Tetherin-mediated Inhibition of Influenza Virus [Microbiology]

    Updated: 2012-06-22 04:00:00
    Skip to main page content Home Current issue Archive Papers in Press Minireviews Classics Reflections Papers of the Week QUICK SEARCH : Author : Keyword : Year : Vol : Page GO Advanced Search Browse the Archive Advertisement Advertisement Influenza Virus Partially Counteracts Restriction Imposed by Tetherin BST-2 Bastien Mangeat Lorris Cavagliotti Martin Lehmann Gustavo Gers-Huber Inderdeep Kaur Yves Thomas Laurent Kaiser and Vincent Piguet 1 From the Department of Dermatology and Wound Healing , Institute of Infection and Immunity , Cardiff University School of Medicine , Cardiff CF14 4XN , Wales , United Kingdom and the Department of Microbiology and Molecular Medicine , Laboratory of Virology , Swiss National Reference Centre of Influenza , and Division of Infectious Diseases ,

  • Survival and Persistence of Non‐spore Forming Biothreat Agents in Water

    Updated: 2012-06-21 21:26:20
    Conclusion: F. tularensis was culturable for at least 21 days if held at 8°C. The remaining non‐spore‐forming bacterial biothreat agents were found to be metabolically active for at least 30 days in water held at 5° or 25°C.Significance and Impact of the Study:  The data can assist public health officials determine the safety of drinking water after contamination with a biothreat agent.© No claim to US Government works. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology)

  • Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates

    Updated: 2012-06-21 04:00:00
    Conclusions: Here we present a transcriptome analysis of the root-colonizing bacterium Bacillus amyloliquefaciens FZB42 in response to maize root exudates. The 302 genes identified as being differentially transcribed are proposed to be involved in interactions of Gram-positive bacteria with plants. (Source: BMC Microbiology - Latest articles)

  • Evolution of French Bordetella pertussis and Bordetella parapertussis isolates: increase of Bordetellae not expressing pertactin

    Updated: 2012-06-21 04:00:00
    : Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Infectious Disease Microbiology Clinical Microbiology and Infection Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Editor's Choice Wiley Job Network ORIGINAL ARTICLE

  • Stenotrophomonas maltophilia flagellin restricts bacterial colonization in BALB/c mouse lung in vivo

    Updated: 2012-06-20 20:18:15
    In conclusion, this study emphasizes the nonspecific protection mediated by flagellin against homologous as well as heterologous bacterium that might be exploited therapeutically to prevent the development of RTI.© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved (Source: FEMS Immunology and Medical Microbiology)

  • Compliance with anti‐H1N1 Vaccine among Health‐Care Workers and General Population

    Updated: 2012-06-20 20:17:10
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Infectious Disease Microbiology Clinical Microbiology and Infection Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles Most Accessed GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Editor's Choice Wiley Job Network Compliance with

  • Identification of a novel role of ESAT‐6‐dependent miR‐155 induction during infection of macrophages with Mycobacterium tuberculosis

    Updated: 2012-06-20 20:16:46
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology Cellular Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Faculty of 1000 Parasitology Virtual Special Issue Posters Virology Virtual Special Issue Wiley Job Network

  • Temperature dependent effect of sub‐lethal levels of cinnamaldehyde on viability and morphology of Escherichia coli

    Updated: 2012-06-20 20:16:18
    ConclusionSub‐lethal levels of cinnamaldehyde at 37 °C delayed multiplication of E. coli cells by causing transient cell elongation, but at 6 °C ≥ 200 mg l−1 cinnamaldehyde was lethal to E. coli.Significance and Impact of the studyInhibition of cold‐induced cell elongation and the enhanced lethal effect of cinnamaldehyde at 6 °C against E. coli O157:H7 suggest cinnamaldehyde may be useful for control of this pathogen at refrigeration temperatures.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Using propidium monoazide to distinguish between viable and nonviable bacteria, MS2, and murine norovirus

    Updated: 2012-06-20 20:16:05
    Conclusions:  These results suggest that a combined PMA‐qPCR assay can be used to measure the viability of bacterial cells and bacteriophage MS2, but not MNV.Significance and Impact of the Study:  PMA‐qPCR could potentially be used to measure the viability of some microorganisms including virus. However, a thorough evaluation should be performed prior to measuring the viability of microorganisms by PMA‐qPCR in a quantitative way.© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Letters in Applied Microbiology)

  • Pomegranate Materials Inhibit Flagellin Gene Expression and Flagellar‐propelled Motility of Uropathogenic Escherichia coli strain CFT073

    Updated: 2012-06-20 20:15:30
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Microbiology Virology FEMS Microbiology Letters Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES FEMS Most Cited Articles FEMS Most Read Articles Virtual Issue : Pathogenic and

  • AI‐2‐mediated signalling in bacteria

    Updated: 2012-06-20 20:15:23
    AbstractSuccess in nature depends upon an ability to perceive and adapt to the surrounding environment. Bacteria are not an exception; they recognize and constantly adjust to changing situations by sensing environmental and self‐produced signals, then altering gene expression accordingly. Autoinducer‐2 (AI‐2) is a signal molecule produced by LuxS, an enzyme found in many bacterial species and thus proposed to enable interspecies communication. Two classes of AI‐2 receptors and many layers and interactions involved in downstream signalling have been identified so far. Though AI‐2 has been implicated in the regulation of numerous niche‐specific behaviours across the bacterial kingdom, interpretation of these results is complicated by the dual role of LuxS in signalling and the ac...

  • Microbial diversity, tolerance, and biodegradation potential of urban wetlands with different input regimes

    Updated: 2012-06-20 16:20:19
    Canadian Journal of Microbiology, Volume 0, Issue 0, Page 887-897, e-First articles. (Source: Canadian Journal of Microbiology)

  • Polymerase chain reaction detection of potentially pathogenic free-living amoebae in dental units

    Updated: 2012-06-20 14:48:33
    Canadian Journal of Microbiology, Volume 0, Issue 0, Page 884-886, e-First articles. (Source: Canadian Journal of Microbiology)

  • Microbiological community analysis of vermicompost tea and its influence on the growth of vegetables and cereals

    Updated: 2012-06-20 07:01:31
    Canadian Journal of Microbiology, Volume 0, Issue 0, Page 836-847, e-First articles. (Source: Canadian Journal of Microbiology)

  • A Bacillus subtilis sensor kinase involved in triggering biofilm formation on the roots of tomato plants

    Updated: 2012-06-20 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Molecular Microbiology Molecular Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES 3D Interactive Images Faculty of 1000 Prizes Awarded by Molecular Microbiology Virtual Special Issues Wiley Job

  • The nitric oxide reductase of enterohaemorrhagic Escherichia coli plays an important role for the survival within macrophages

    Updated: 2012-06-20 04:00:00
    SummaryIn enterohaemorrhagic Escherichia coli (EHEC) O157, there are two types of anaerobic nitric oxide (NO) reductase genes, an intact gene (norV) and a 204 bp deletion gene (norVs). Epidemiological analysis has revealed that norV‐type EHEC are more virulent than norVs‐type EHEC. Thus, to reveal the role of NO reductase during EHEC infection, we constructed isogenic norV‐type and norVs‐type EHEC mutant strains. Under anaerobic conditions, the norV‐type EHEC was protected from NO‐mediated growth inhibition, while the norVs‐type EHEC mutant strain was not, suggesting that NorV of EHEC was effective in the anaerobic detoxification. We then investigated the role of NO reductase within macrophages. The norV‐type EHEC produced a lower NO level within macrophages compared with...

  • A network involving Rho‐type GTPases, a paxillin and a formin homologue regulates spore length and spore wall integrity in the filamentous fungus Ashbya gossypii

    Updated: 2012-06-20 04:00:00
    , Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Molecular Microbiology Molecular Microbiology Early View Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES 3D Interactive Images Faculty of 1000 Prizes Awarded by Molecular Microbiology Virtual Special Issues Wiley

  • Proteolytic profiling and comparative analyses of active trypsin-like serine peptidases in preimaginal stages of Culex quinquefasciatus

    Updated: 2012-06-20 04:00:00
    This paper reports on the biochemical characterization and comparative analysis of multiple, highly active trypsin-like serine peptidases in egg, larval and pupal stages of Culex quinquefasciatus. Pre-imaginal stages express a complex proteolytic profile with optimal activity at alkaline pH and over a wide range of temperature. The comparative approach used here allowed the detection of differences in the expression of active peptidases in the analyzed stages and permitted the assignment of specific trypsin activity to each stage. Image: Trypsin-like serine peptidases' profiles exhibited by egg, larva and pupa of Cx. quinquefasciatus (Source: Parasites and Vectors)

  • Molecular analysis of volatile metabolites released specifically by Staphylococcus aureus and Pseudomonas aeruginosa

    Updated: 2012-06-20 04:00:00
    Conclusions: The results obtained provide strong evidence that the detection and perhaps even identification of bacteria could be achieved by determination of characteristic volatile metabolites, supporting the clinical use of breath-gas analysis as non-invasive method for early detection of bacterial lung infections. (Source: BMC Microbiology - Latest articles)MedWorm Sponsor Message: Please have a look at this new site driven by MedWorm: The Breast Cancer Daily

  • Developing vaccines in the era of genomics: a decade of Reverse Vaccinology

    Updated: 2012-06-20 04:00:00
    AbstractVaccines have a significant impact on public health, and vaccinology in the era of genomics is taking advantage of new technologies to tackle diseases for which vaccine development has so far been unsuccessful. Almost all existing vaccines were developed based on traditional vaccinology methods, which relied on empirical screening of a few candidates at a time based on known features of the pathogen. However, the ability to sequence a pathogen’s genome provides access to its entire antigenic repertoire. As such, genomics has catalysed a shift in vaccine development towards sequence‐based ‘Reverse Vaccinology’ approaches, which use high‐throughput in silico screening of the entire genome of a pathogen to identify genes that encode proteins with the attributes of good vacci...

  • Synergism between hydrogen peroxide and seventeen acids against six bacterial strains

    Updated: 2012-06-20 04:00:00
    ConclusionsSynergistic combinations of disinfectants were revealed, among them the promising hydrogen peroxide / formic acid combination.Significance and impact of the studyA rapid screening method was proposed and used to reveal the synergistic potential of disinfectants and/or sanitizers combinations.© 2012 Anses Fougères Laboratory Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Competitive effect of commensal fecal bacteria from growing swine fed chlortetracycline–supplemented feed on β–hemolytic Escherichia coli strains with multiple antimicrobial resistance plasmids

    Updated: 2012-06-20 04:00:00
    ConclusionsThere was no significant effect on the fecal microflora, due to the inclusion of chlortetracycline, in the swine diets, that affected the growth of E. coli in the competition studies employed. Furthermore, these results suggest the cost of maintaining plasmids in these E. coli strains had little influence on survivability.Significance and Impact of StudyMutations that led to antimicrobial resistance may have a greater impact on survivability than multiple plasmid carriage.© No claim to US Government works. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Reducing the variability between constant‐depth film fermenter experiments when modelling oral biofilm

    Updated: 2012-06-20 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Applied Microbiology Journal of Applied Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues Virtual Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL Society Information News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES Virtual Issue on Biodefence Virtual Issue on Campylobacter

  • Analysis and characterization of cultivable extremophilic hydrolytic bacterial community in heavy‐metal‐contaminated soils from the Atacama Desert and their biotechnological potentials

    Updated: 2012-06-20 04:00:00
    ConclusionsThe diversity found in the cultivable bacterial community analyzed is more limited than that detected in other ecological studies due to the restrictive conditions used in the screening. The dominant bacteria were Firmicutes and particularly, species related to the genus Bacillus.Significance and Impact of StudyThis study is focused on the characterization of extremophilic hydrolytic bacteria, providing candidates as a source of novel enzymes with biotechnological applications.© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology (Source: Journal of Applied Microbiology)

  • Evidence for Roles of the Escherichia coli Hda Protein Beyond RIDA

    Updated: 2012-06-20 04:00:00
    AbstractThe ATP‐bound form of the Escherichia coli DnaA protein binds ‘DnaA boxes’ present in the origin of replication (oriC) and operator sites of several genes, including dnaA, to coordinate their transcription with initiation of replication. The Hda protein, together with the ß sliding clamp, stimulates the ATPase activity of DnaA via a process termed Regulatory Inactivation of DnaA (RIDA), to regulate the activity of DnaA in DNA replication. Here, we used the mutant dnaN159 strain, which expresses the ß159 clamp protein, to gain insight into how the actions of Hda are coordinated with replication. Elevated expression of Hda impeded growth of the dnaN159 strain in a Pol II‐ and Pol IV‐dependent manner, suggesting a role for Hda managing the actions of these Pols. In a wild ...

  • The magnetosome membrane protein, MmsF, is a major regulator of magnetite biomineralization in Magnetospirillum magneticum AMB‐1

    Updated: 2012-06-20 04:00:00
    SummaryMagnetotactic bacteria (MTB) use magnetosomes, membrane bound crystals of magnetite or greigite, for navigation along geomagnetic fields. In Magnetospirillum magneticum sp. AMB‐1, and other MTB, a magnetosome gene island (MAI) is essential for every step of magnetosome formation. An 8‐gene region of the MAI encodes several factors implicated in control of crystal size and morphology in previous genetic and proteomic studies. We show that these factors play a minor role in magnetite biomineralization in vivo. In contrast, MmsF, a previously uncharacterized magnetosome membrane protein encoded within the same region plays a dominant role in defining crystal size and morphology and is sufficient for restoring magnetite synthesis in the absence of the other major biomineralization c...

  • Activity of the tetrapyrrole regulator CrtJ is controlled by oxidation of a redox active cysteine located in the DNA binding domain

    Updated: 2012-06-20 04:00:00
    AbstractCrtJ from Rhodobacter capsulatus is a regulator of genes involved in the biosynthesis of heme, bacteriochlorophyll, carotenoids as well as structural proteins of the light harvesting‐II complex. Fluorescence anisotropy based DNA‐binding analysis demonstrates that oxidized CrtJ exhibits ∼20‐fold increase in binding affinity over that of reduced CrtJ. Liquid chromatography electrospray tandem mass spectrometric analysis using DAz‐2, a sulfenic acid (‐SOH)‐specific probe, demonstrates that exposure of CrtJ to oxygen or to hydrogen peroxideleads to significant accumulation of a sulfenic acid derivative of Cys420 which is located in the helix‐turn‐helix (HTH) motif. In vivo labeling with 4‐ (3‐azidopropyl)cyclohexane‐1, 3 ‐dione (DAz‐2) shows that Cys420 also...

  • Hemophore functions revisited

    Updated: 2012-06-20 04:00:00
    summayIron is essential for most organisms, including bacteria. It is a key element in oxidoreduction reactions, due to the wide range of Fe2+/Fe3+ redox potentials dependent on its ligands. Free ferrous ion is harmful in the presence of oxygen, generating hydroxyl radicals by dihydrogen peroxide decomposition© 2012 Blackwell Publishing Ltd (Source: Molecular Microbiology)

  • The ABC transporter MalFGK2 sequesters the MalT transcription factor at the membrane in the absence of cognate substrate

    Updated: 2012-06-20 04:00:00
    Skip to Main Content Home Help PUBLICATIONS BROWSE BY SUBJECT RESOURCES ABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED FORGOTTEN PASSWORD INSTITUTIONAL LOGIN Home Microbiology Virology Molecular Microbiology Molecular Microbiology Accepted Articles Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current Issue All Issues FIND ARTICLES Early View Accepted Articles GET ACCESS Subscribe Renew FOR CONTRIBUTORS OnlineOpen Author Guidelines Submit an Article ABOUT THIS JOURNAL News Overview Editorial Board Permissions Advertise Contact SPECIAL FEATURES 3D Interactive Images Faculty of 1000 Prizes Awarded by Molecular Microbiology Virtual Special Issues

  • Repeated Exposure to Triclosan Reduces Virulence in S. aureus

    Updated: 2012-06-19 17:16:00
    Repeated laboratory exposures to triclosan reduced susceptibility to antibiotics in Staphylococcus aureus, but probably not sufficiently to render commonly used antibiotics ineffective, according to a paper in the June 2012 issue of the journal Antimicrobial Agents and Chemotherapy. It also generated less virulent, less fit “small colony variants” of the pathogen. Read more... (Source: American Society for Microbiology)

  • Changing Pigs’ Diets Alters the Gut Microbiota

    Updated: 2012-06-19 16:38:00
    Including chicory in cereal-based diets of pigs results in profound changes in gut micro-environment, morphology, and microbial population of pigs, according to a study in the June 2012 Applied and Environmental Microbiology.  Read more... (Source: American Society for Microbiology)

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