On the viability of Escherichia coli cells lacking DNA topoisomerase I
Updated: 2012-02-28 04:00:00
Conclusions:
Our data show unambiguously that E. coli cells are not viable in the absence of DNA topoisomerase I without the presence of compensatory mutations. Furthermore, our data suggest that the accumulation of R-loops is not the primary reason for the severe growth defect of cells lacking topoisomerase I, which is in contrast to the current literature. Potential reasons for this discrepancy are discussed. (Source: BMC Microbiology - Latest articles)