Large segments of DNA can vary in copy number between individuals. Such copy number variations (CNVs) contribute greatly to genetic diversity and are also thought to be associated with susceptibility or resistance to some diseases, including cancer. Simple Copy Number Determination with Reference Query Pyrosequencing (RQPS), featured in the September issue of Cold Spring [...]
Jim Maher, Ph.D. Mark McNiven, Ph.D. Dev Mukhopadhyay, Ph.D. _______________________________________
(ScienceDaily) – Using a 3-D visualization method called X-ray crystallography, Song Tan at Pe
Leaders of the Roy J. Carver Charitable Trust of Muscatine, Iowa, University of Iowa Health Care and the UI Foundation have announced a $2 million gift from the Carver Charitable Trust that will establish a core facilities operational endowment for proteomics in the UI Roy J. and Lucille A. Carver College of Medicine.
Proteomics involves the [...]
Leaders of the Roy J. Carver Charitable Trust of Muscatine, Iowa, University of Iowa Health Care and the UI Foundation have announced a $2 million gift from the Carver Charitable Trust that will establish the Roy J. Carver Chair in Biochemistry.
Charles Brenner, Ph.D., professor and head of biochemistry, will serve as the Roy J. Carver [...]Improvements in automation and acquisition time have made the microscope a viable platform for performing hundreds of concurrent parallel experiments. Using these sorts of tools, it is now possible to run high-throughput screens for protein function and interaction in living cells, examining dynamic cellular processes to distinguish between primary and secondary phenotypes, and to [...]
Steven Lentz (right), MD, PhD, Professor, Department of Internal Medicine, has been named the new director of the Medical Scientist Training Program (MSTP) in the UI Carver College of Medicine. Dr. Lentz previously served as the associate director of the program. Pamela Geyer, PhD, Professor of Biochemistry, who directed the program from 1999 to 2006, [...]
Masquerade parties are a lot of fun. Inspiration for costumes comes from all kinds of sources. At th
Algae Anatomy Biochemistry and Biotechnology DOWNLOAD FULLImmunoprecipitation is a commonly used technique for isolating and purifying a protein of interest. An antibody for the protein is incubated with a cell extract, and the resulting antibody/antigen complex is pulled out of solution. The method used for preparation of the cell extract can be critical for the experiment’s success. The [...]Producing recombinant proteins in bacterial hosts is a widely-used laboratory procedure. But generating a large yield of protein is often challenging. Getting enough raw material for experiments can be a time-consuming and frustrating process. In the August issue of Cold Spring Harbor Protocols, Jianjun Wang and colleagues present a method for Preparation [...]Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme. ZFNs can be used to generate targeted genomic deletions of large segments of DNA in a wide variety of cell types and organisms. In the August issue [...]While it is possible to analyze the global lipid composition of a cell, a deeper understanding of what lipids are doing within that cell is more difficult to come by. Though the lipid components may be known, finding their exact position, how dynamically they change location, and how rapidly they are metabolized presents an [...]The zebrafish (Danio rerio) has rapidly become a favored model organism for studying developmental biology. One of the most commonly used methods for genetic manipulation in the zebrafish is the delivery of plasmids or oligonucleotides to cells within the living embryo via electroporation. When cells are exposed to brief electrical fields, transient membrane destabilization [...]Cold Spring Harbor Laboratory Press’ new Drosophila Neurobiology laboratory manual covers the three main approaches taught in the CSHL course: studying neural development, recording and imaging the nervous system, and studying behavior. The featured electrophysiology paper is part of the recording/imaging section, while the second featured article in the July issue of Cold Spring [...]