While it is possible to analyze the global lipid composition of a cell, a deeper understanding of what lipids are doing within that cell is more difficult to come by. Though the lipid components may be known, finding their exact position, how dynamically they change location, and how rapidly they are metabolized presents an [...]
I am a first year med student with only a little previous knowledge of chemistry and therefore was aThe zebrafish (Danio rerio) has rapidly become a favored model organism for studying developmental biology. One of the most commonly used methods for genetic manipulation in the zebrafish is the delivery of plasmids or oligonucleotides to cells within the living embryo via electroporation. When cells are exposed to brief electrical fields, transient membrane destabilization [...]Cold Spring Harbor Laboratory Press’ new Drosophila Neurobiology laboratory manual covers the three main approaches taught in the CSHL course: studying neural development, recording and imaging the nervous system, and studying behavior. The featured electrophysiology paper is part of the recording/imaging section, while the second featured article in the July issue of Cold Spring [...]
Download Discuss the module activity here… 16.390488 120.593448Since the early days of the 20th century and Thomas Hunt Morgan’s famous “Fly Room” at Columbia University, the fruit fly Drosophila melanogaster has been at the forefront of biological research. The powerful arsenal of experimental methods developed for this model organism is now being used to tackle one of the great scientific challenges of [...]
University of Iowa Health Care leaders have announced the appointment of Curt Sigmund, Ph.D., as professor and head of the Department of Pharmacology in the University of Iowa Roy J. and Lucille A. Carver College of Medicine.
Sigmund, who is currently a faculty member in the departments of internal medicine and molecular [...]The June issue of Cold Spring Harbor Protocols includes an early preview of CSHL Press’ forthcoming RNA: A Laboratory Manual. Protocols covering basic RNA techniques are now available, including methods for purification of RNA by by SDS Solubilization and Phenol Extraction and by Using TRIzol (TRI Reagent), Ethanol Precipitation of RNA and the Use [...]
Researchers at the University of Iowa Carver College of Medicine and University of Nebraska Medical Center (UNMC) have created a three-dimensional picture of an important protein that is involved in how HIV — the virus responsible for AIDS — is produced inside human cells. The picture may help researchers design drugs [...]The dynamic nature of biological processes has long been difficult to document, as researchers have been limited to static studies based on fixed specimens. Methods like immunocytochemistry or in situ hybridization can only provide accurate information on one organism at one particular time point. As Scott Fraser has remarked, it’s akin to trying [...]
Ernesto Fuentes, Ph.D., assistant professor of biochemistry at the University of Iowa Carver College of Medicine, has received a five-year $1 million CAREER award from the National Science Foundation (NSF).
The grant is NSF’s most prestigious award for junior faculty who exemplify the role of scholar-teacher by integrating outstanding research with excellent teaching.
The funding will support [...]The rapid pace of technological progress in biological imaging has provided great insight into the processes of embryonic development. But for higher organisms with opaque eggs or internal development, optical access to the embryo is limited. While various embryonic culture methods are available, vertebrate development is best studied in an intact embryo model, [...]