Antibiotics as Enzymes
Updated: 2010-06-19 08:04:41
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The June issue of Cold Spring Harbor Protocols includes an early preview of CSHL Press’ forthcoming RNA: A Laboratory Manual. Protocols covering basic RNA techniques are now available, including methods for purification of RNA by by SDS Solubilization and Phenol Extraction and by Using TRIzol (TRI Reagent), Ethanol Precipitation of RNA and the Use [...]
The dynamic nature of biological processes has long been difficult to document, as researchers have been limited to static studies based on fixed specimens. Methods like immunocytochemistry or in situ hybridization can only provide accurate information on one organism at one particular time point. As Scott Fraser has remarked, it’s akin to trying [...]
The rapid pace of technological progress in biological imaging has provided great insight into the processes of embryonic development. But for higher organisms with opaque eggs or internal development, optical access to the embryo is limited. While various embryonic culture methods are available, vertebrate development is best studied in an intact embryo model, [...]
The generation of transgenic plants can be a lengthy and difficult process. Transient expression assays have been developed as faster and more convenient alternatives for investigating gene function. These assays often take advantage of the ability of Agrobacterium to transfer foreign DNA into plant cells with intact cell walls. Agrobacterium-mediated transformation is, however, [...]
Using a promoter that can drive expression at an appropriate level is crucial in designing constructs for gene expression. Promoters can be tested via transient or stable transfection. But transfection efficiency in such assays can be low, so promoters are commonly fused to heterologous reporter genes that encode enzymes that can be [...]
Hollis Cline, Ph.D. will speak as part of the College’s Distinguished Biomedical Scholar Lecture Series on Thursday, May 20. Cline’s talk is entitled “Building Brain Circuits,” and will be held in Urmila Sahai Seminar Room (2117 MERF) at 4 pm. The talk is sponsored by the Department of Molecular Physiology and Biophysics.
Cline received her Ph.D. [...]
In an effort to increase communication with investigators, the Tissue Procurement Core Facility has launched a new wiki portal. The new TissueWiki portal will serve as a way for investigators to leave comments for the Facility, ask questions, or communicate with Facility staff or other investigators utilizing the core’s services.
Comments posted on the TissueWiki system [...]
Congratulations to the 2010 Research Week poster session winners! Both poster sessions were again a large success, featuring over 100 participants each day. Winners were recognized by College and University leadership April 14 during the annual Research Week awards ceremony.
Below, view award winners from the Carver College of Medicine, Institute for Clinical and Translational Science, [...]
In an effort to make Research Week seminars available to all campus investigators, the 2010 talks are now available for viewing online. Many of the videos utilize a new lecture-capture system recently implemented on the CCOM campus. The system offers video footage of speakers along side a screen capture of Powerpoint slides and other visual [...]
The large size and external development of the frog Xenopus laevis make it an ideal system for in vivo imaging of dynamic cellular activity. Xenopus embryos are amenable to simple genetic manipulation techniques including knockdowns and misexpression, as well as transgenesis. The ease of collecting large numbers of embryos and the larger size of [...]