Identified new genes responsible for obesity
Updated: 2010-04-30 07:00:42
Hey Everyone! I was wandering around checking the latest news and look what I’ve found; according to
April’s issue of Cold Spring Harbor Protocols includes instructions for Rapid Coomassie Blue Staining of Protein Gels. This method is an adaptation of the conventional Coomassie staining protocol described in Staining Proteins in Gels with Coomassie Blue. Coomassie Brilliant Blue R250 (CBR-250) is the most commonly used dye for visualizing proteins because of [...]
The other blog where I write, The Scholarly Kitchen, has been nominated for a Webby, a fairly prestigious award in the online world. Since the winner is determined by the voting public, and since we’re up against some seriously stiff competition (including the NY Times and Wall Street Journal), your help would be greatly [...]
While 454-based pyrosequencing has led to great advances, an intrinsic artifact of the process leads to artificial over-representation of more than 10% of the original DNA sequencing templates. This is particularly problematic in metagenomic studies, where the abundance of any sequence in a dataset is often used for comparative community analysis. It’s important [...]
Pentose Phosphate Pathway …anabolic pathway that utilizes the 6 carbons of glucose to generate 5 car
Dates, times and titles for this year’s Health Sciences Research Week presentations have been finalized. This year’s event includes presentations by six leading researchers in the field of genetics and two poster sessions. Events are set to take place Tuesday, April 13 through April 15.
See complete schedule below:
Tuesday, April 13
12:00 – 1:00 pm | 2117 [...]
Individuals presenting posters during Research Week are reminded of the following:
Posters should be posted no later than 1:00 pm in the MERF Atrium. Poster and board number assignments are available online at the links below.
Student Session- Tuesday, April 13
Faculty, Staff, Postdoc Session- Wednesday, April 14
Presenters should check in at the registration table.
Presenters with posters in [...]
Kevin Campbell, Ph.D., professor and head of molecular physiology and biophysics at the UI Carver College of Medicine, has received a $517,814 grant from the Muscular Dystrophy Association (MDA) to continue his research on muscle biology.
Campbell, who also is a Howard Hughes Medical Institute investigator, will study muscular dystrophies caused by defects in a cellular [...]
Neurons are organized into anatomical and functional groups called “circuits”. The activity of these circuits is traditionally monitored using conventional electrophysiological techniques. But some cells, such as the submandibular ganglia, are difficult to impale for intracellular recordings. Instead, viral vectors can be used to deliver fluorescent calcium sensors for detecting activity in a [...]
The goal of tissue engineering is to recapitulate healthy human organs and tissue structures in culture, and then transplant them into patients, where they are fully integrated. This is a complicated process, and the use of high-throughput imaging systems that allow researchers to directly monitor transplanted tissues in live animals over time is important [...]
The recent explosion in the availability and variety of fluorescent proteins, new organic dyes and quantum dots has been a driving force in the growing use of Total Internal Reflection Fluorescence Microscopy (TIRFM). TIRFM only illuminates molecules that are within a thin volume near the coverslip surface of a specimen and not those deeper [...]
The baculovirus expression vector system has been widely used to produce proteins originating from both prokaryotic and eukaryotic sources. It offers easy cloning techniques and abundant viral propagation, and since it is based on an insect cell environment, it provides eukaryotic posttranslational modification machinery. Surface modifications of the viral capsid enable specific targeting. Such [...]
The use of recombinant proteins, antibodies, small molecules, or nucleic acids as affinity reagents is a simple yet powerful strategy to study the protein/bait interactions that drive biological processes. Analysis via mass spectrometry rather than western blotting extends the identification of interactors, often allowing detection of thousands of proteins from complex mixtures. But this [...]